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https://hdl.handle.net/1822/91908
Título: | Physicochemical properties of a bioactive polysaccharide film from cassia grandis with immobilized collagenase from Streptomyces parvulus (DPUA/1573) |
Autor(es): | Ferreira, Kétura Cardoso, Kethylen Costa, Romero Brandão Martins, Joana T. Botelho, C. M. Neves, Anna Nascimento, Thiago Batista, Juanize Ferreira, Éverton Damasceno, Fernando Sales-Conniff, Armanda Albuquerque, Wendell Porto, Ana Teixeira, J. A. |
Palavras-chave: | Polysaccharide film Collagenase Streptomyces parvulus Skin healing |
Data: | 29-Mai-2024 |
Editora: | MDPI AG |
Revista: | Cosmetics |
Citação: | Ferreira, Kétura; Cardoso, Kethylen; Costa, Romero Brandão; Martins, Joana T.; Botelho, Cláudia M.; Neves, Anna; Nascimento, Thiago; Batista, Juanize; Ferreira, Éverton; Damasceno, Fernando; Sales-Conniff, Armanda; Albuquerque, Wendell; Porto, Ana; Teixeira, José A., Physicochemical properties of a bioactive polysaccharide film from cassia grandis with immobilized collagenase from Streptomyces parvulus (DPUA/1573). Cosmetics, 11(3), 86, 2024 |
Resumo(s): | (1) Background: Polysaccharide films are promising vehicles for the delivery of bioactive agents such as collagenases, as they provide controlled release at the wound site, facilitating tissue regeneration. This study aimed to investigate the physicochemical properties of Cassia grandis polysaccharide films with immobilized collagenase from Streptomyces parvulus (DPUA/1573). (2) Methods: Galactomannan was extracted from Cassia grandis seeds for film production with 0.8% (w/v) galactomannan and 0.2% (v/v) glycerol with or without collagenases. The films underwent physical-chemical analyses: Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), color and opacity (luminosity-L*, green to red-a*, yellow to blue-b*, opacity-Y%), moisture content, water vapor permeability (WVP), thickness, contact angle, and mechanical properties. (3) Results: The results showed similar FTIR spectra to the literature, indicating carbonyl functional groups. Immobilizing bioactive compounds increased surface roughness observed in SEM. TGA indicated a better viability for films with immobilized S. parvulus enzymes. Both collagenase-containing and control films exhibited a bright-yellowish color with slight opacity (Y%). Mechanical tests revealed decreased rigidity in PCF (25%) and SCF (41%) and increased deformability in films with the immobilized bioactive compounds, PCF (234%) and SCF (295%). (4) Conclusions: Polysaccharide-based films are promising biomaterials for controlled composition, biocompatibility, biodegradability, and wound healing, with a potential in pharmacological applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/91908 |
DOI: | 10.3390/cosmetics11030086 |
ISSN: | 2079-9284 |
Versão da editora: | www.mdpi.com/journal/cosmetics |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_57889_1.pdf | 3,73 MB | Adobe PDF | Ver/Abrir |