Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/90382
Título: | 2,4,5-Triaminopyrimidines as blue fluorescent probes for cell viability monitoring: synthesis, photophysical properties, and microscopy applications |
Autor(es): | Gonçalves, Jorge M. Gonçalves, João N. D. Sousa, Luís F. Rodrigues, L. R. Correia-de-Sá, Paulo Coutinho, Paulo J. G. Castanheira, Elisabete M. S. Oliveira, Rui Pedro Soares de Dias, Alice |
Palavras-chave: | 2,4,5-Triaminopyrimidines Fluorescent probes |
Data: | 15-Fev-2024 |
Editora: | Royal Society of Chemistry |
Revista: | Organic & Biomolecular Chemistry |
Citação: | Gonçalves JM, Gonçalves JND, Sousa LF, Rodrigues LR, Correia-de-Sá P, Coutinho PJG, Castanheira EMS, Oliveira R, Dias AM. 2,4,5-Triaminopyrimidines as blue fluorescent probes for cell viability monitoring: synthesis, photophysical properties, and microscopy applications. Org Biomol Chem. 2024 Mar 13;22(11):2252-2263. doi: 10.1039/d4ob00092g. |
Resumo(s): | Monitoring cell viability is critical in cell biology, pathology, and drug discovery. Most cell viability assays are cell-destructive, time-consuming, expensive, and/or hazardous. Herein, we present a series of newly synthesized 2,4,5-triaminopyrimidine derivatives able to discriminate between live and dead cells. To our knowledge, these compounds are the first fluorescent nucleobase analogues (FNAs) with cell viability monitoring potential. These new fluorescent molecules are synthesized using highly efficient and cost- effective methods and feature unprecedented photophysical properties (longer absorption and emission wavelengths, environment-sensitive emission, and unprecedented brightness within FNAs). Using a live– dead Saccharomyces cerevisiae cell and theoretical assays, the fluorescent 2,4,5-triaminopyrimidine derivatives were found to specifically accumulate inside dead cells by interacting with dsDNA grooves, thus paving the way for the emergence of novel and safe fluorescent cell viability markers emitting in the blue region. As the majority of commercially available viability dyes emit in the green to red region of the visible spectrum, these novel markers might be useful to meet the needs of blue markers for co-staining combinations. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/90382 |
DOI: | 10.1039/d4ob00092g |
Versão da editora: | https://pubs.rsc.org/en/content/articlepdf/2024/ob/d4ob00092g |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series CBMA - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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d4ob00092g.pdf | 2,2 MB | Adobe PDF | Ver/Abrir |