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https://hdl.handle.net/1822/88772
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Campo DC | Valor | Idioma |
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dc.contributor.author | Carvalho, Duarte Nuno | por |
dc.contributor.author | Dani, Sophie | por |
dc.contributor.author | Sotelo, Carmen G. | por |
dc.contributor.author | Perez-Martin, Ricardo I. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Silva, Tiago H. | por |
dc.contributor.author | Gelinsky, Michael | por |
dc.date.accessioned | 2024-02-15T08:31:21Z | - |
dc.date.available | 2024-02-15T08:31:21Z | - |
dc.date.issued | 2023-08 | - |
dc.date.submitted | 2024-02 | - |
dc.identifier.citation | Carvalho D. N., Dani S., Sotelo C. G., Perez-Martin R. I., Reis R. L., Silva T. H., Gelinsky M. Assessing non-synthetic crosslinkers in biomaterial inks based on polymers of marine origin to increase the shape fidelity in 3D extrusion printing, Biomedical Materials, Vol. 18, Issue 5, pp. 055017, doi:10.1088/1748-605X/acecec, 2023 | por |
dc.identifier.issn | 1748-605X | por |
dc.identifier.uri | https://hdl.handle.net/1822/88772 | - |
dc.description.abstract | In the past decade, there has been significant progress in 3D printing research for tissue engineering (TE) using biomaterial inks made from natural and synthetic compounds. These constructs can aid in the regeneration process after tissue loss or injury, but achieving high shape fidelity is a challenge as it affects the constructâ s physical and biological performance with cells. In parallel with the growth of 3D bioprinting approaches, some marine-origin polymers have been studied due to their biocompatibility, biodegradability, low immunogenicity, and similarities to human extracellular matrix components, making them an excellent alternative to land mammal-origin polymers with reduced disease transmission risk and ethical concerns. In this research, collagen from shark skin, chitosan from squid pens, and fucoidan from brown algae were effectively blended for the manufacturing of an adequate biomaterial ink to achieve a printable, reproducible material with a high shape fidelity and reticulated using four different approaches (phosphate-buffered saline, cell culture medium, 6% CaCl2, and 5 mM Genipin). Materials characterization was composed by filament collapse, fusion behavior, swelling behavior, and rheological and compressive tests, which demonstrated favorable shape fidelity resulting in a stable structure without deformations, and interesting shear recovery properties around the 80% mark. Additionally, live/dead assays were conducted in order to assess the cell viability of an immortalized human mesenchymal stem cell line, seeded directly on the 3D printed constructs, which showed over 90% viable cells. Overall, the Roswell Park Memorial Institute cell culture medium promoted the adequate crosslinking of this biopolymer blend to serve the TE approach, taking advantage of its capacity to hamper pH decrease coming from the acidic biomaterial ink. While the crosslinking occurs, the pH can be easily monitored by the presence of the indicator phenol red in the cell culture medium, which reduces costs and time. | por |
dc.description.sponsorship | The authors would like to acknowledge to Portuguese Foundation of Science and Technology (FCT) for PhD fellowship (D N Carvalho) under the scope of the doctoral program Tissue Engineering, Regenerative Medicine and Stem Cells, Ref. PD/BD/143044/2018, and The German Research Foundation (S Dani). The authors also like to acknowledge Dr Julio Maroto from the Fundación CETMAR for kindly providing collagen from shark skin, and Roi Vilela from PESCANOVA S A, Spain, for the kind offer of squid pens for chitin/chitosan extraction. The Article Processing Charges (APC) for making the article Open Access were funded by the joint publication funds of the TU Dresden, including Carl Gustav Carus Faculty of Medicine, and the SLUB Dresden as well as the Open Access Publication Funding of the German Reearch Foundation (DFG). | por |
dc.language.iso | eng | por |
dc.publisher | IOP Publishing | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBD%2F143044%2F2018/PT | por |
dc.rights | openAccess | por |
dc.subject | Bioprinting | por |
dc.subject | Highly viscous bioink | por |
dc.subject | Marine biomaterials | por |
dc.subject | Marine biopolymers | por |
dc.subject | Microextrusion | por |
dc.subject | Plotting | por |
dc.title | Assessing non-synthetic crosslinkers in biomaterial inks based on polymers of marine origin to increase the shape fidelity in 3D extrusion printing | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://iopscience.iop.org/article/10.1088/1748-605X/acecec | por |
dc.comments | http://3bs.uminho.pt/node/21040 | por |
oaire.citationIssue | 5 | por |
oaire.citationVolume | 18 | por |
dc.date.updated | 2024-02-06T09:39:23Z | - |
dc.identifier.doi | 10.1088/1748-605X/acecec | por |
dc.identifier.pmid | 37531962 | por |
sdum.journal | Biomedical Materials | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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21040-96-carvalho2023-colchifuinks-3dprinting.pdf | 2,68 MB | Adobe PDF | Ver/Abrir |