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https://hdl.handle.net/1822/88099
Título: | Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction |
Autor(es): | Marques, Bianca Nunes, Rafaela Araújo-Rodrigues, Helena Pintado, Manuela Pereira, Ricardo N. Teixeira, José A. Rocha, Cristina M.R. |
Palavras-chave: | Autohydrolysis Hydrothermal treatments Protein hydrolysis Meat by-products Circular economy |
Data: | 2024 |
Editora: | Springer Nature |
Revista: | Food and Bioprocess Technology |
Citação: | Marques, Bianca; Nunes, R.; Araújo-Rodrigues, Helena; Pintado, Manuela; Pereira, Ricardo N.; Teixeira, José A.; Rocha, Cristina M. R., Solubilization and hydrolysis of porcine coagulated blood protein using sub-critical solvent extraction. Food and Bioprocess Technology, 17, 123-137, 2024 |
Resumo(s): | Pork represents a major fraction of the meat consumed worldwide but only 30% of the blood generated in slaughterhouses is re-used as raw material for food and feed. Innovative technologies and efficient processing strategies capable of generating added-value products from it are now attracting attention. In this study, the hydrolysis of porcine coagulated blood using sub-critical solvent extraction was investigated. Biomass was hydrolyzed using different temperatures (120210 °C), applying only water (sub-critical water; SCW) or water with a low concentration of alkali (0.1 mol L1 NaOH) and different reaction times (3090 min). Resultant hydrolysates were analyzed for crude and soluble protein, peptide profile, and bioactivity by combining protein quantification, antioxidant activity, and fast protein liquid chromatography measurements. Results showed that increasing temperature increases the degree of hydrolysis and that the addition of NaOH enhances the solubilization of peptides with high molecular weights. Also, hydrolysates showed interesting antioxidant activity, being 60 min the time of reaction with best antioxidant activity. Nevertheless, using only water (SCW) as solvent, without chemical additives, allows the delivering of interesting protein-based bioactive fractions. Sub-critical solvent treatment of porcine blood resulted in added-value fractions with potential bioactivities through a simple and environmentally friendly process. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/88099 |
DOI: | 10.1007/s11947-023-03111-3 |
ISSN: | 1935-5130 |
Versão da editora: | http://www.springer.com/food+science/journal/11947 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56337_1.pdf | 1,91 MB | Adobe PDF | Ver/Abrir |