Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/87709
Título: | Exploring the use of hexadecane by Yarrowia lipolytica: Effect of dissolved oxygen and medium supplementation |
Autor(es): | Miranda, Sílvia Cristiana Martins Lopes, Marlene Belo, Isabel |
Palavras-chave: | Hexadecane Dissolved oxygen Yarrowia lipolytica Microbial lipids Enzymes |
Data: | 20-Jan-2024 |
Editora: | Elsevier 1 |
Revista: | Journal of Biotechnology |
Citação: | Miranda, S. M., Lopes, M., & Belo, I. (2024, January). Exploring the use of hexadecane by Yarrowia lipolytica: Effect of dissolved oxygen and medium supplementation. Journal of Biotechnology. Elsevier BV. http://doi.org/10.1016/j.jbiotec.2023.12.006 |
Resumo(s): | This work aimed to evaluate the effect of medium composition and volumetric oxygen transfer coefficient (kLa) on Y. lipolytica growth and production of microbial lipids and enzymes from hexadecane. In the stirred tank bioreactor, increasing kLa from 11 h 1 to 132 h 1 improved the hexadecane assimilation rate, biomass concentration, and lipids synthesis (0.90 gL 1). A cost-effective hexadecane-based medium supplemented with corn steep liquor and a low amount of ammonium sulfate boosted lipids production up to 2.1 gL1, composed of palmitic, palmitoleic, oleic, and linoleic acids. The unsaturated/saturated fraction was dependent on the C/N ratio. Lipids of Y. lipolytica CBS 2075 are promising feedstock for animal feed, food additives, or the biodiesel industry. Simultaneous synthesis of extracellular lipase and protease from hexadecane was observed, which is a new feature that was not previously reported. The highest enzyme activity was obtained at the highest C/N ratio conditions. These results open new perspectives on the application of Y. lipolytica-based cultures for the biotransformation of hexadecane-polluted streams into valuable compounds, fulfilling an interesting strategy towards the circular economy concept. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/87709 |
DOI: | 10.1016/j.jbiotec.2023.12.006 |
ISSN: | 0168-1656 |
Versão da editora: | http://www.elsevier.com/locate/issn/01681656 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56539_1.pdf | 2 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons