Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/84802
Título: | Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regeneration |
Autor(es): | Alves, Ana L. Carvalho, Ana C. Machado, Inês Diogo, Gabriela S. Fernandes, Emanuel M. Castro, Vânia Isabel Baptista Pires, Ricardo A. Vázquez, José A. Pérez-Martín, Ricardo I. Alaminos, Miguel Reis, R. L. Silva, Tiago H. |
Palavras-chave: | Marine biomaterials Gelatin Codfish GelMA Keratocytes Cornea |
Data: | 4-Jan-2023 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | Bioengineering |
Citação: | Alves, A.L.; Carvalho, A.C.; Machado, I.; Diogo, G.S.; Fernandes, E.M.; Castro, V.I.B.; Pires, R.A.; Vázquez, J.A.; Pérez-Martín, R.I.; Alaminos, M.; Reis, R.L.; Silva, T.H. Cell-Laden Marine Gelatin Methacryloyl Hydrogels Enriched with Ascorbic Acid for Corneal Stroma Regeneration. Bioengineering 2023, 10, 62. https://doi.org/10.3390/bioengineering10010062 |
Resumo(s): | Corneal pathologies from infectious or noninfectious origin have a significant impact on the daily lives of millions of people worldwide. Despite the risk of organ rejection or infection, corneal transplantation is currently the only effective treatment. Finding safe and innovative strategies is the main goal of tissue-engineering-based approaches. In this study, the potential of gelatin methacryloyl (GelMA) hydrogels produced from marine-derived gelatin and loaded with ascorbic acid (as an enhancer of the biological activity of cells) was evaluated for corneal stromal applications. Marine GelMA was synthesized with a methacrylation degree of 75%, enabling effective photocrosslinking, and hydrogels with or without ascorbic acid were produced, encompassing human keratocytes. All the produced formulations exhibited excellent optical and swelling properties with easy handling as well as structural stability and adequate degradation rates that may allow proper extracellular matrix remodeling by corneal stromal cells. Formulations loaded with 0.5 mg/mL of ascorbic acid enhanced the biological performance of keratocytes and induced collagen production. These results suggest that, in addition to marine-derived gelatin being suitable for the synthesis of GelMA, the hydrogels produced are promising biomaterials for corneal regeneration applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/84802 |
DOI: | 10.3390/bioengineering10010062 |
e-ISSN: | 2306-5354 |
Versão da editora: | https://www.mdpi.com/2306-5354/10/1/62 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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bioengineering-10-00062.pdf | 5,63 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons