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https://hdl.handle.net/1822/83397
Título: | Inhibition of enzyme hydrolysis of cellulose by phenols from hydrothermally pretreated sugarcane straw |
Autor(es): | Michelin, Michele Ximenes, Eduardo Polizeli, Maria de Lourdes T. M. Ladisch, Michael R. |
Palavras-chave: | Lignocellulose Enzyme inhibition Advanced biorefineries Phenolics Deactivation Inhibition Cellulase |
Data: | Mai-2023 |
Editora: | Elsevier 1 |
Revista: | Enzyme and Microbial Technology |
Citação: | Michelin, Michele; Ximenes, Eduardo; Polizeli, Maria de Lourdes T. M.; Ladisch, Michael R., Inhibition of enzyme hydrolysis of cellulose by phenols from hydrothermally pretreated sugarcane straw. Enzyme and Microbial Technology, 166(110227), 2023 |
Resumo(s): | Relatively few studies have addressed the characterization of sugarcane straw (SCS) for production fermentable sugars through enzyme hydrolysis. Straw is a major co-product of the sugarcane harvest in Brazil that has potential to sustainably increase cellulosic feedstocks in Brazil by 50%. Pretreatment of 10% w/v straw with liquid hot water (LHW) at 180°C for 50min (severity, So, of 4.05), solubilizes hemicellulose, preserves glucan, and generates 4.49g/L soluble phenolic compounds in the resulting liquid. Extracts from washing pretreated solids with hot excess water followed by acetone resulted in 1.10 and 0.83g/L phenolics, respectively. Acetone-derived extracts were more inhibitory and decreased glucose yield for enzyme hydrolysis of Solka Floc (a lignin-free cellulose) by 42%. In comparison, pretreated straw washed with hot water or acetone was readily hydrolyzed to 92% and 97% by cellulase enzyme. Hydrothermally treated SCS has the potential to provide a valuable and added source of fermentable sugars suitable for bioprocessing into biofuels and bioproducts when cellulase enzyme inhibitors are removed after pretreatment. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/83397 |
DOI: | 10.1016/j.enzmictec.2023.110227 |
ISSN: | 0141-0229 |
e-ISSN: | 1879-0909 |
Versão da editora: | http://www.journals.elsevier.com/enzyme-and-microbial-technology/ |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_56116_1.pdf Acesso restrito! | 1,33 MB | Adobe PDF | Ver/Abrir |