Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/78469

TítuloProfiling microglia in a mouse model of Machado-Joseph disease
Autor(es)Campos, Ana Bela
Silva, Sara Carina Duarte
Fernandes, B.
Neves, Sofia Pereira
Marques, Fernanda
Castro, Andreia Cristiana Teixeira
Carvalho, Andreia Alexandra Neves
Fernandes, Daniela Monteiro
Portugal, Camila Cabral
Socodato, Renato
Summavielle, Teresa
Ambrósio, António Francisco
Relvas, João Bettencourt
Maciel, P.
Palavras-chaveMicroglia
Machado-Joseph disease
Cell morphology
RNA-sequencing
Machine learning
Data23-Jan-2022
EditoraMultidisciplinary Digital Publishing Institute (MDPI)
RevistaBiomedicines
CitaçãoCampos, A.B.; Duarte-Silva, S.; Fernandes, B.; das Neves, S.P.; Marques, F.; Teixeira-Castro, A.; Neves-Carvalho, A.; Monteiro-Fernandes, D.; Portugal, C.C.; Socodato, R.; Summavielle, T.; Ambrósio, A.F.; Relvas, J.B.; Maciel, P. Profiling Microglia in a Mouse Model of Machado–Joseph Disease. Biomedicines 2022, 10, 237. https://doi.org/10.3390/biomedicines10020237
Resumo(s)Microglia have been increasingly implicated in neurodegenerative diseases (NDs), and specific disease associated microglia (DAM) profiles have been defined for several of these NDs. Yet, the microglial profile in Machado–Joseph disease (MJD) remains unexplored. Here, we characterized the profile of microglia in the CMVMJD135 mouse model of MJD. This characterization was performed using primary microglial cultures and microglial cells obtained from disease-relevant brain regions of neonatal and adult CMVMJD135 mice, respectively. Machine learning models were implemented to identify potential clusters of microglia based on their morphological features, and an RNA-sequencing analysis was performed to identify molecular perturbations and potential therapeutic targets. Our findings reveal morphological alterations that point to an increased activation state of microglia in CMVMJD135 mice and a disease-specific transcriptional profile of MJD microglia, encompassing a total of 101 differentially expressed genes, with enrichment in molecular pathways related to oxidative stress, immune response, cell proliferation, cell death, and lipid metabolism. Overall, these results allowed us to define the cellular and molecular profile of MJD-associated microglia and to identify genes and pathways that might represent potential therapeutic targets for this disorder.
TipoArtigo
URIhttps://hdl.handle.net/1822/78469
DOI10.3390/biomedicines10020237
e-ISSN2227-9059
Versão da editorahttps://www.mdpi.com/2227-9059/10/2/237
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:BUM - MDPI

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Este trabalho está licenciado sob uma Licença Creative Commons Creative Commons

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