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https://hdl.handle.net/1822/77840
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Campo DC | Valor | Idioma |
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dc.contributor.author | Alheib, Omar | por |
dc.contributor.author | Silva, Lucília Pereira | por |
dc.contributor.author | Morais, Alain da Silva | por |
dc.contributor.author | Mesquita, Katia A. | por |
dc.contributor.author | Pirraco, Rogério P. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Correlo, V. M. | por |
dc.date.accessioned | 2022-05-23T15:54:08Z | - |
dc.date.issued | 2022-03 | - |
dc.date.submitted | 2022-05 | - |
dc.identifier.citation | Alheib O., da Silva L. P., Morais A., Mesquita K. A., Pirraco R. P., Reis R. L., Correlo V. M. Injectable laminin-biofunctionalized gellan gum hydrogels loaded with myoblasts for skeletal muscle regeneration, Acta Biomaterialia, Vol. 143, pp. 282-294, doi:10.1016/j.actbio.2022.03.008, 2022 | por |
dc.identifier.issn | 1742-7061 | por |
dc.identifier.uri | https://hdl.handle.net/1822/77840 | - |
dc.description.abstract | Moderate muscular injuries that exceed muscular tissue's auto-healing capacity are still a topic of noteworthy concern. Tissue engineering appeared as a promising therapeutic strategy capable of overcoming this unmet clinical need. To attain such goal, herein we propose an in situ-crosslinking gellan gum (GG)-based hydrogel tethered with a skeletal muscle-inspired laminin-derived peptide RKRLQVQLSIRTC(Q) and encapsulated with skeletal muscle cells (SMCs). Pre-hydrogel solutions presented decreasing shear viscosity with increasing shear rate and shear stress, and required low forces for extrusion, validating their injectability. The GGDVS hydrogel was functionalized with Q-peptide with 30% of efficiency. C2C12 were able to adhere to the developed hydrogel, remained living and spreading 7 days post-encapsulation. Q-peptide release studies indicated that 25% of the unbound peptide can be released from the hydrogels up to 7 days, dependent on the hydrogel formulation. Treatment of a chemically-induced muscular lesion in mice with an injection of C2C12-laden hydrogels improved myogenesis, primarily promoted by the C2C12. In accordance, a high density of myoblasts (α-SA+ and MYH7+) were localized in tissues treated with the C2C12 (alone or encapsulated in the hydrogel). α-SA protein levels were significantly increased 8 weeks post-treatment with C2C12-laden hydrogels and MHC protein levels were increased in all experimental groups 4 weeks post-treatment, in relation to the SHAM. Neovascularization and neoinnervation was also detected in the defects. Altogether, this study indicates that C2C12-laden hydrogels hold great potential for skeletal muscle regeneration. Statement of significance We developed an injectable gellan gum-based hydrogel for delivering C2C12 into localized myopathic model. The gellan gum was biofunctinalized with laminin-derived peptide to mimic the native muscular ECM. In addition, hydrogel was physically tuned to mimic the mechanical properties of native tissue. To the best of our knowledge, this formula was used for the first time under the context of skeletal muscle tissue regeneration. The injectability of the developed hydrogel provided non-invasive administration method, combined with a reliable microenvironment that can host C2C12 with nominal inflammation, indicated by the survival and adhesion of encapsulated cells post-injection. The treatment of skeletal muscle defect with the cell-laden hydrogel approach significantly enhanced the regeneration of localized muscular trauma. | por |
dc.description.sponsorship | The authors acknowledge the financial support from the project JUSThera (NORTE-01-0145-FEDER-000055), and from Portuguese Foundation for Science and Technology (FCT) for the PhD grant FCT PD/BD/128090/2016 under TERM program PD/59/2013 (OA) and when eligible, by COMPETE 2020 FEDER funds, under the Scientific Employment Stimulus - Individual Call (CEEC Individual) - 2020.01541.CEECIND/ CP1600/CT0024 (LdS). The authors would also like to acknowledge Dr. Reem Sweid for image segmentation using Matlab, Teresa Oliveira for her support in histology. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.relation | NORTE-01-0145-FEDER-000055 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBD%2F128090%2F2016/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/CEEC IND 3ed/2020.01541.CEECIND%2FCP1600%2FCT0024/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | C2C12 | por |
dc.subject | Gellan gum | por |
dc.subject | Hydrogel | por |
dc.subject | Skeletal muscle tissue engineering | por |
dc.title | Injectable laminin-biofunctionalized gellan gum hydrogels loaded with myoblasts for skeletal muscle regeneration | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S1742706122001398 | por |
dc.comments | http://3bs.uminho.pt/node/20763 | por |
oaire.citationStartPage | 282 | por |
oaire.citationEndPage | 294 | por |
oaire.citationVolume | 143 | por |
dc.date.updated | 2022-05-16T10:01:33Z | - |
dc.identifier.eissn | 1878-7568 | por |
dc.identifier.doi | 10.1016/j.actbio.2022.03.008 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 35278687 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Acta Biomaterialia | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20763-1-s20-s1742706122001398-main-3.pdf Acesso restrito! | 4,93 MB | Adobe PDF | Ver/Abrir |