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https://hdl.handle.net/1822/77011
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Campo DC | Valor | Idioma |
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dc.contributor.author | Monteiro, R. | por |
dc.contributor.author | Magalhães, Andreia Patrícia Alves | por |
dc.contributor.author | Pereira, Maria Olívia | por |
dc.contributor.author | Sousa, Ana Margarida | por |
dc.date.accessioned | 2022-04-19T14:36:01Z | - |
dc.date.issued | 2021-08 | - |
dc.identifier.citation | Monteiro, R., Magalhães, A. P., Pereira, M. O., & Sousa, A. M. (2021). Long-term coexistence of Pseudomonas aeruginosa and Staphylococcus aureus using an in vitro cystic fibrosis model. Future Microbiology, 16(12), 879-893. doi: 10.2217/fmb-2021-0025 | por |
dc.identifier.issn | 1746-0913 | por |
dc.identifier.uri | https://hdl.handle.net/1822/77011 | - |
dc.description.abstract | Aim: To investigate the role of pre-established Staphylococcus aureus on Pseudomonas aeruginosa adaptation and antibiotic tolerance. Materials & methods: Bacteria were cultured mimicking the sequential pattern of lung colonization and exposure to ciprofloxacin. Results: In the absence of ciprofloxacin exposure, S. aureus and P. aeruginosa coexisted supported by the physicochemical characteristics of the artificial sputum medium. S. aureus had no role in P. aeruginosa tolerance against ciprofloxacin and did not select P. aeruginosa small-colony variants during antibiotic treatment. rhlR and psqE were downregulated after the contact with S. aureus indicating that P. aeruginosa attenuated its virulence potential. Conclusion: P. aeruginosa and S. aureus can cohabit in cystic fibrosis airway environment for long-term without significant impact on P. aeruginosa adaptation and antibiotic tolerance. | por |
dc.description.sponsorship | This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit. The authors also acknowledge COMPETE2020 and FCT for the project POCI-01-0145-FEDER-029841, FCT for the PhD grants of R Monteiro (grant number 2020.04988.BD) and AP Magalhaes (grant number SFRH/BD/132165/2017) and for Scientific Employment Stimulus 2017 (CEECIND/01507/2017) provided to AM Sousa. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed | por |
dc.language.iso | eng | por |
dc.publisher | Future Medicine | por |
dc.relation | UID/BIO/04469/2020 | por |
dc.relation | SFRH/BD/132165/2017 | por |
dc.relation | CEECIND/01507/2017 | por |
dc.rights | restrictedAccess | por |
dc.subject | Coexistence | por |
dc.subject | Cystic fibrosis | por |
dc.subject | In vitro sequential growth | por |
dc.subject | Interspecies interaction | por |
dc.subject | Pseudomonas aeruginosa | por |
dc.subject | Staphylococcus aureus | por |
dc.title | Long-term coexistence of Pseudomonas aeruginosa and Staphylococcus aureus using an in vitro cystic fibrosis model | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.futuremedicine.com/doi/10.2217/fmb-2021-0025 | por |
dc.comments | CEB54607 | por |
oaire.citationStartPage | 879 | por |
oaire.citationEndPage | 893 | por |
oaire.citationIssue | 12 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationVolume | 16 | por |
dc.date.updated | 2022-04-19T13:44:59Z | - |
dc.identifier.eissn | 1746-0921 | por |
dc.identifier.doi | 10.2217/fmb-2021-0025 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 34319132 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Future Microbiology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_54607_1.pdf Acesso restrito! | 3,9 MB | Adobe PDF | Ver/Abrir |