Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75761
Título: | Exploitation of a klebsiella bacteriophage receptor-binding protein as a superior biorecognition molecule |
Autor(es): | Nogueira, Catarina Pires, Diana P. Monteiro, Rodrigo Santos, Sílvio Roberto Branco Carvalho, Carla M. |
Palavras-chave: | Bacteriophage Receptor-binding protein (RBP) Klebsiella pneumoniae Diagnosis Hospital acquired infections (HAIs) |
Data: | 7-Out-2021 |
Editora: | American Chemical Society |
Revista: | ACS Infectious Diseases |
Citação: | Nogueira, Catarina; Pires, Diana P.; Monteiro, Rodrigo; Santos, Sílvio Roberto Branco; Carvalho, Carla M., Exploitation of a Klebsiella bacteriophage receptor-binding protein as a superior biorecognition molecule. ACS Infectious Diseases, 7(11), 3077-3087, 2021 |
Resumo(s): | Klebsiella pneumoniae is a Gram-negative bacterium that has become one of the leading causes of life-threatening healthcare-associated infections (HAIs), including pneumonia and sepsis. Moreover, due to its increasingly antibiotic resistance, K. pneumoniae has been declared a global top priority concern. The problem of K. pneumoniae infections is due, in part, to the inability to detect this pathogen rapidly and accurately and thus to treat patients within the early stages of infections. The success in bacterial detection is greatly dictated by the biorecognition molecule used, with the current diagnostic tools relying on expensive probes often lacking specificity and/or sensitivity. (Bacterio)phage receptor-binding proteins (RBPs) are responsible for the recognition and adsorption of phages to specific bacterial host receptors and thus present high potential as biorecognition molecules. In this study, we report the identification and characterization of a novel RBP from the K. pneumoniae phage KpnM6E1 that presents high specificity against the target bacteria and high sensitivity (80\%) to recognize K. pneumoniae strains. Moreover, adsorption studies validated the role of gp86 in the attachment to bacterial receptors, as it highly inhibits (86\%) phage adsorption to its Klebsiella host. Overall, in this study, we unravel the role and potential of a novel Klebsiella phage RBP as a powerful tool to be used coupled with analytical techniques or biosensing platforms for the diagnosis of K. pneumoniae infections. |
Tipo: | Artigo |
Descrição: | The Supporting Information is available free of charge at: https://pubs.acs.org/doi/10.1021/acsinfecdis.1c00366 |
URI: | https://hdl.handle.net/1822/75761 |
DOI: | 10.1021/acsinfecdis.1c00366 |
ISSN: | 2373-8227 |
Versão da editora: | https://pubs.acs.org/doi/10.1021/acsinfecdis.1c00366 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54917_1.pdf Acesso restrito! | 2,75 MB | Adobe PDF | Ver/Abrir |