Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75661
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Campo DC | Valor | Idioma |
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dc.contributor.author | Guimarães, Diana Isabel Pereira | por |
dc.contributor.author | Lager, Franck | por |
dc.contributor.author | Renault, Gilles | por |
dc.contributor.author | Guezguez, Jamil | por |
dc.contributor.author | Burnet, Michael | por |
dc.contributor.author | Cunha, Joana | por |
dc.contributor.author | Cavaco-Paulo, Artur | por |
dc.contributor.author | Nogueira, Eugénia | por |
dc.date.accessioned | 2022-01-25T14:04:28Z | - |
dc.date.available | 2022-01-25T14:04:28Z | - |
dc.date.issued | 2022-01-21 | - |
dc.identifier.citation | Guimarães, D.; Lager, F.; Renault, G.; Guezguez, J.; Burnet, M.; Cunha, J.; Cavaco-Paulo, A.; Nogueira, E. Folate-Targeted Liposomal Formulations Improve Effects of Methotrexate in Murine Collagen-Induced Arthritis. Biomedicines 2022, 10, 229. https://doi.org/10.3390/biomedicines10020229 | por |
dc.identifier.issn | 2227-9059 | por |
dc.identifier.uri | https://hdl.handle.net/1822/75661 | - |
dc.description.abstract | Methotrexate (MTX) is first-line therapy for the treatment of rheumatoid arthritis (RA), however, its use may be limited by side effects notably post-injection malaise. When patients are intolerant or become unresponsive, second-line or antibody therapy may be indicated. A folate-targeted liposomal formulation of MTX (FL-MTX) is tropic to arthritic paws and prevents the onset of collagen-induced arthritis (CIA) in the mouse. We optimized the drug-to-lipid molar ratio to 0.15 and demonstrated the therapeutic efficacy of this form at 2 mg/kg MTX intraperitoneal (i.p.) twice a week. These improved liposomes were present in inflamed joints in proportion to the degree of swelling of the paw and bone remodeling activity. FL-MTX had lower hepatic and renal elimination of MTX than the free substance. FL-MTX provided equivalent results when given i.p. or subcutaneous (s.c.) and FL-MTX 2 mg/kg (drug/lipid 0.15), twice weekly, was similar to or more effective than 35 mg/kg MTX (same route and schedule) in reducing the incidence and swelling in the murine CIA model. These results suggest that FL-MTX is a more potent nanotherapeutic formulation than free MTX treatment. Its potential benefits for patients may include reduced frequency of treatment and lower overall doses for a given response. | por |
dc.description.sponsorship | This work has received funding from the European Union Horizon 2020 research and innovation program under grant agreement NMP-06-2015-683356 FOLSMART. This study was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145- FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020— Programa Operacional Regional do Norte. Diana Guimarães (SFRH/BD/140321/2018) holds a scholarship from FCT. Nuclear imaging was performed at CNRS, TAAM UPS44, In Vivo Imaging Centre for Preclinical and Translational Research CIPA, 45071, Orléans, France. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.relation | UIDB/04469/2020 | por |
dc.relation | SFRH/BD/140321/2018 | por |
dc.rights | openAccess | por |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Liposomes | por |
dc.subject | Methotrexate | por |
dc.subject | Folate-targeting | por |
dc.subject | Rheumatoid arthritis | por |
dc.subject | Collagen-induced arthritis | por |
dc.title | Folate-targeted liposomal formulations improve effects of methotrexate in murine collagen-induced arthritis | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/2227-9059/10/2/229 | por |
dc.comments | CEB55189 | por |
oaire.citationStartPage | 229 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 10 | por |
dc.date.updated | 2022-01-25T11:09:28Z | - |
dc.identifier.doi | 10.3390/biomedicines10020229 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomedicines | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55189_1.pdf | 2,66 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons