Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75475
Título: | Effective elastin-like recombinamers coating on poly(vinylidene) fluoride membranes for mesenchymal stem cell culture |
Autor(es): | Guillot-Ferriols, M. del Barrio, A. Costa, Carlos Miguel Silva Lanceros-Méndez, S. Rodríguez-Cabello, J. C. Gómez Ribelles, J. L. Santos, M. Gallego Ferrer, G. |
Palavras-chave: | Poly(vinylidene) fluoride Piezoelectricity Elastin-like recombinamers RGD Layer-by-layer Mesenchymal stem cells |
Data: | 2021 |
Editora: | Elsevier 1 |
Revista: | European Polymer Journal |
Citação: | Guillot-Ferriols, M., del Barrio, A., Costa, C. M., Lanceros Méndez, S., Rodríguez-Cabello, J. C., Gómez Ribelles, J. L., . . . Gallego Ferrer, G. (2021). Effective elastin-like recombinamers coating on poly(vinylidene) fluoride membranes for mesenchymal stem cell culture. European Polymer Journal, 146, 110269. doi: https://doi.org/10.1016/j.eurpolymj.2021.110269 |
Resumo(s): | Bone’s inherent piezoelectricity is a key factor in regulating bone growth and mesenchymal stem cells (MSCs) fate towards the osteogenic lineage. The piezoelectric polymer poly(vinylidene) fluoride (PVDF) was thus used to manufacture electroactive membranes by means of non-solvent induced phase separation (NIPS), producing porous membranes with approximately 90% of γ-phase for MSCs culture. The combination of the porous surface and PVDF hydrophobicity hinders cell adhesion and requires a coating to improve cell culture conditions. A layer-by-layer (LbL) method was used to deposit elastin-like recombinamers (ELRs) containing RGD sequences applying click cross-linking chemistry. ELRs potential was confirmed by comparing traditional fibronectin adsorption with ELRs LbL on PVDF electroactive membranes. Porcine bone marrow MSCs preferred ELRs-coated surfaces, which enhanced initial cell adhesion and improved proliferation after 7 days. These findings lead to new possibilities for regenerative therapies in the area of bone tissue engineering, offering the advantages of MSC commitment towards the osteogenic lineage by applying electro-mechanical stimulation on electroactive substrates. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/75475 |
DOI: | 10.1016/j.eurpolymj.2021.110269 |
ISSN: | 0014-3057 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0014305721000033 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CDQuim - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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43.pdf | Effective elastin-like recombinamers coating on poly(vinylidene) fluoride membranes for mesenchymal stem cell culture | 1,41 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons