Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/72765
Título: | Successive domain rearrangements underlie the evolution of a regulatory module controlled by a small interfering peptide |
Autor(es): | Raimundo, João Alexandre Pereira Sobral, Rómulo Sacramento Laranjeira, Sara Costa, Maria Manuela Ribeiro |
Palavras-chave: | MYB RADIALIS DIVARICATA DRIF Protein evolution Protein-protein interaction Domain earrangement Molecular antagonism Small interfering peptide Flower asymmetry Antirrhinum majus Domain rearrangement |
Data: | 2018 |
Editora: | Oxford University Press |
Revista: | Molecular Biology and Evolution |
Citação: | Raimundo, J., Sobral, R., Laranjeira, S., & Costa, M. M. R. (2018). Successive domain rearrangements underlie the evolution of a regulatory module controlled by a small interfering peptide. Molecular biology and evolution, 35(12), 2873-2885 |
Resumo(s): | The establishment of new interactions between transcriptional regulators increases the regulatory diversity that drives phenotypic novelty. To understand how such interactions evolve, we have studied a regulatory module (DDR) composed by three MYB-like proteins: DIVARICATA (DIV), RADIALIS (RAD), and DIV-and-RAD-Interacting Factor (DRIF). The DIV and DRIF proteins form a transcriptional complex that is disrupted in the presence of RAD, a small interfering peptide, due to the formation of RAD-DRIF dimers. This dynamic interaction result in a molecular switch mechanism responsible for the control of distinct developmental processes in plants. Here, we have determined how the DDR regulatory module was established by analyzing the origin and evolution of the DIV, DRIF, and RAD protein families and the evolutionary history of their interactions. We show that duplications of a pre-existing MYB domain originated the DIV and DRIF protein families in the ancestral lineage of green algae, and, later, the RAD family in seed plants. Intraspecies interactions between the MYB domains of DIV and DRIF proteins are detected in green algae, whereas the earliest evidence of an interaction between DRIF and RAD proteins occurs in the gymnosperms, coincident with the establishment of the RAD family. Therefore, the DDR module evolved in a stepwise progression with the DIV-DRIF transcription complex evolving prior to the antagonistic RAD-DRIF interaction that established the molecular switch mechanism. Our results suggest that the successive rearrangement and divergence of a single protein domain can be an effective evolutionary mechanism driving new protein interactions and the establishment of novel regulatory modules. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/72765 |
DOI: | 10.1093/molbev/msy178 |
ISSN: | 0737-4038 |
Versão da editora: | https://academic.oup.com/mbe/article/35/12/2873/5092475?login=true |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CBFP - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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msy178.pdf Acesso restrito! | 1,2 MB | Adobe PDF | Ver/Abrir |