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https://hdl.handle.net/1822/72167
Título: | Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applications |
Autor(es): | Radhouani, Hajer Correia, Susana Gonçalves, Cristiana Reis, R. L. Oliveira, J. M. |
Palavras-chave: | Biomedical applications Chemical cross-linking Hydrogel Kefiran Methacrylation |
Data: | Abr-2021 |
Editora: | MDPI |
Revista: | Polymers |
Citação: | Radhouani H., Correia S., Gonçalves C., Reis R. L., Oliveira J. M. Synthesis and Characterization of Biocompatible Methacrylated Kefiran Hydrogels: Towards Tissue Engineering Applications, Polymers, Vol. 13, Issue 8, pp. 1342, doi:10.3390/polym13081342, 2021 |
Resumo(s): | Hydrogel application feasibility is still limited mainly due to their low mechanical strength and fragile nature. Therefore, several physical and chemical cross-linking modifications are being used to improve their properties. In this research, methacrylated Kefiran was synthesized by reacting Kefiran with methacrylic anhydride (MA). The developed MA-Kefiran was physicochemically characterized, and its biological properties evaluated by different techniques. Chemical modification of MA-Kefiran was confirmed by 1H-NMR and FTIR and GPC-SEC showed an average Mw of 793 kDa (PDI 1.3). The mechanical data obtained revealed MA-Kefiran to be a pseudoplastic fluid with an extrusion force of 11.21 ± 2.87 N. Moreover, MA-Kefiran 3D cryogels were successfully developed and fully characterized. Through micro-CT and SEM, the scaffolds revealed high porosity (85.53 ± 0.15%) and pore size (33.67 ± 3.13 μm), thick pore walls (11.92 ± 0.44 μm) and a homogeneous structure. Finally, MA-Kefiran revealed to be biocompatible by presenting no hemolytic activity and an improved cellular function of L929 cells observed through the AlamarBlue® assay. By incorporating methacrylate groups in the Kefiran polysaccharide chain, a MA-Kefiran product was developed with remarkable physical, mechanical, and biological properties, resulting in a promising hydrogel to be used in tissue engineering and regenerative medicine applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/72167 |
DOI: | 10.3390/polym13081342 |
ISSN: | 2073-4360 |
Versão da editora: | https://www.mdpi.com/2073-4360/13/8/1342 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20519-radhouani-et-al-2021.pdf | 1,88 MB | Adobe PDF | Ver/Abrir |