Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/71044
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Campo DC | Valor | Idioma |
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dc.contributor.author | Gaspar, Carlos | por |
dc.contributor.author | Rolo, Joana | por |
dc.contributor.author | Cerca, Nuno | por |
dc.contributor.author | Palmeira-de-Oliveira, Rita | por |
dc.contributor.author | Martinez-de-Oliveira, José | por |
dc.contributor.author | Palmeira-de-Oliveira, Ana | por |
dc.date.accessioned | 2021-03-26T21:02:01Z | - |
dc.date.available | 2021-03-26T21:02:01Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Gaspar, Carlos; Rolo, Joana; Cerca, Nuno; Palmeira-de-Oliveira, Rita; Martinez-de-Oliveira, José; Palmeira-de-Oliveira, Ana, Dequalinium chloride effectively disrupts bacterial vaginosis (BV) Gardnerella spp. biofilms. Pathogens, 10(3), 261, 2021 | por |
dc.identifier.issn | 20760817 | por |
dc.identifier.uri | https://hdl.handle.net/1822/71044 | - |
dc.description.abstract | Bacterial vaginosis (BV) is the most frequent vaginal infection worldwide. It is caused by the overgrowth of anaerobic vaginal pathogens such as Gardnerella spp. BV has been associated with the occurrence of dense multispecies biofilms on the vaginal mucosa. Treatment of biofilm-associated infections such as BV is challenging. In this study, we have tested the role of a quaternary ammonium compound, dequalinium chloride (DQC), in the eradication of Gardnerella spp. biofilms. The effects of the test substance on the biomass and the metabolic activity of the biofilm of Gardnerella spp. were assessed in vitro using a microtiter plate assay. In addition, the effect of DQC on the Gardnerella spp. biofilm was further assessed by using scanning electron microscopy and confocal laser scanning microscopy. The results showed that DQC was particularly effective in the destruction of BV-associated Gardnerella spp. biotypes, impacting both their biomass and metabolic activity. In addition, the disruption of biofilm architecture was evident and was probably caused by multiple mechanisms of action. We conclude that DQC is an antibiofilm agent and is able to efficiently destroy Gardnerella spp. BV-associated biofilms. Therefore, it is a valid option for BV therapy and has the potential to prevent BV recurrences. | por |
dc.description.sponsorship | This work was supported by national funds from Fundação para a Ciência e Tecnologia (FCT), Portugal (Project UIDB/00709/2020), and by the European Regional Development Fund (ERDF), under the Portugal 2020 Program, through the Regional Operational Program of the Center (Centro2020), through the Project with the reference UIDB/00709/2020. C. Gaspar was supported by fellowship SFRH/BDE/112920/2015 from FCT; J. Rolo was supported by fellowship SFRH/BPD/115145/2016 from FCT. The human resources, consumables, and reagents, as well as publication charges, were partially funded by Medinova, Switzerland. All the results achieved are reported in this document (including negative results), and no member of Medinova interfered in the experimental design or discussion of results. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.relation | UIDB/00709/2020 | por |
dc.relation | UIDB/00709/2020 | por |
dc.relation | SFRH/BDE/112920/2015 | por |
dc.relation | SFRH/BPD/115145/2016 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | bacterial vaginosis | por |
dc.subject | dequalinium chloride | por |
dc.subject | biofilm | por |
dc.subject | treatment | por |
dc.subject | recurrence prevention | por |
dc.title | Dequalinium chloride effectively disrupts bacterial vaginosis (BV) Gardnerella spp. biofilms | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.mdpi.com/journal/pathogens | por |
dc.comments | CEB54247 | por |
oaire.citationStartPage | 261 | por |
oaire.citationEndPage | 11 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 10 | por |
dc.date.updated | 2021-02-27T10:38:59Z | - |
dc.identifier.doi | 10.3390/pathogens10030261 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Pathogens | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54247_1.pdf | 2,12 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons