Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/69035
Título: | Attomolar label-free detection of DNA hybridization with electrolyte-gated Graphene field-effect transistors |
Autor(es): | Campos, Rui Borme, Jérôme Guerreiro, Joana Rafaela Machado, George Cerqueira, M. F. Petrovykh, Dmitri Y. Alpuim, P. |
Palavras-chave: | Biosensor DNA EGFET (electrolyte-gated field-effect transistor) Graphite Models, Molecular Molecular Conformation Nucleic Acid Hybridization Polymorphism, Single Nucleotide Surface Properties Limit of Detection Transistors, Electronic Graphene Planar technology Receded gate transistor Surface functionalization |
Data: | Jan-2019 |
Editora: | ACS |
Revista: | ACS Sensors |
Citação: | Campos, R., Borme, J., Guerreiro, J. R., Machado Jr, G., et. al. (2019). Attomolar label-free detection of DNA hybridization with electrolyte-gated graphene field-effect transistors. ACS sensors, 4(2), 286-293 |
Resumo(s): | In this work, we develop a field-effect transistor with a two-dimensional channel made of a single graphene layer to achieve label-free detection of DNA hybridization down to attomolar concentration, while being able to discriminate a single nucleotide polymorphism (SNP). The SNP-level target specificity is achieved by immobilization of probe DNA on the graphene surface through a pyrene-derivative heterobifunctional linker. Biorecognition events result in a positive gate voltage shift of the graphene charge neutrality point. The graphene transistor biosensor displays a sensitivity of 24 mV/dec with a detection limit of 25 aM: the lowest target DNA concentration for which the sensor can discriminate between a perfect-match target sequence and SNP-containing one. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/69035 |
DOI: | 10.1021/acssensors.8b00344 |
ISSN: | 2379-3694 |
Versão da editora: | https://pubs.acs.org/doi/10.1021/acssensors.8b00344 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CDF - CEP - Artigos/Papers (with refereeing) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
ACS_Sensors_Campos et al.pdf | Accepted manuscript | 876,61 kB | Adobe PDF | Ver/Abrir |
Supporting Information for Publication_Campos et al.pdf | Supporting Information | 1,93 MB | Adobe PDF | Ver/Abrir |