Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/67995
Título: | Otoliths-composed gelatin/sodium alginate scaffolds for bone regeneration |
Autor(es): | Valido, Daisy Pereira Júnior, Wilson Déda Gonçalves de Andrade, Maria Eliane Rezende, Allan Andrade de Andrade de Carvalho, Felipe Mendes de Lima, Renata das Graças Gomes Trindade, Gabriela de Alcântara Campos, Caio Oliveira, Ana Maria Santos de Souza, Eloísa Portugal Barros Silva Soares Frank, Luiza Abrahão Guterres, Silvia Stanisçuaski Sussuchi, Eliana Midori Matos, Charlene Regina Santos Polloni, André de Souza Araújo, Adriano Antunes Padilha, Francine Ferreira Severino, Patrícia Souto, Eliana B. de Albuquerque Júnior, Ricardo Luiz Cavalcanti |
Palavras-chave: | Otoliths Bone regeneration Tissue scaffolds Tissue engineering |
Data: | 2020 |
Editora: | Springer |
Revista: | Drug Delivery and Translational Research |
Citação: | Valido, Daisy Pereira; Júnior, Wilson Déda Gonçalves; de Andrade, Maria Eliane; Rezende, Allan Andrade; de Andrade de Carvalho, Felipe Mendes; de Lima, Renata; das Graças Gomes Trindade, Gabriela; de Alcântara Campos, Caio; Oliveira, Ana Maria Santos; de Souza, Eloísa Portugal Barros Silva Soares; Frank, Luiza Abrahão; Guterres, Silvia Stanisçuaski; Sussuchi, Eliana Midori; Matos, Charlene Regina Santos; Polloni, André; de Souza Araújo, Adriano Antunes; Padilha, Francine Ferreira; Severino, Patrícia; Souto, Eliana; de Albuquerque Júnior, Ricardo Luiz Cavalcanti, Otoliths-composed gelatin/sodium alginate scaffolds for bone regeneration. Drug Delivery and Translational Research, 10, 1716-1728, 2020 |
Resumo(s): | Evidence that otoliths, mineral-rich limestone concrescences present in the inner ear of bone fishes, can accelerate bone formation in vivo has been previously reported. The goal of this work was the development, characterization, and evaluation of the cytocompatibility of otoliths-incorporated sodium alginate and gelatin scaffolds. Cynoscion acoupaderived otoliths were characterized by X-ray fluorescence spectrometry (FRX), particle size, free lime, and weight loss by calcination. Furthermore, otoliths were incorporated into sodium alginate (ALG/OTL-s) or gelatin (GEL/OTL-s) scaffolds, previously developed by freeze-drying. Then, the scaffolds were characterized by thermogravimetric analysis (TGA/DTG), differential scanning calorimetry (DSC), infrared spectroscopy with Fourier transform (FTIR), swelling tests, and scanning electron microscopy (SEM). Cytotoxicity assays were run against J774.G8 macrophages and MC3T3-E1 osteoblasts. Data obtained from TGA/DTG, DSC, and FTIR analyses confirmed the interaction between otoliths and the polymeric scaffolds. SEM showed the homogeneous porous 3D structure rich in otolith micro-fragments in both scaffolds. Swelling of the GEL/OTL-s (63.54±3.0%) was greater than of ALG/OTL-s (13.36±9.9%) (p<0.001). The viability of J774.G8 macrophages treated with both scaffolds was statistically similar to the group treated with DMEM only (p>0.05) and significantly higher than that treated with Triton-X (p<0.01) at 72 h. Both scaffolds showed approximately 100% growth of MC3T3-E1 osteoblasts by 24 h, similarly to control (p>0.05). However, by 48 h, only ALG/OTL-s showed growth similar to control (p>0.05), whereas GEL/OTL showed a significantly lower growth index (p<0.05). In conclusion, the physicochemical profiles suggest proper interaction between the otoliths and the two developed polymeric 3D scaffolds. Moreover, both materials showed cytocompatibility with J774.G8 macrophages but the growth of MC3T3-E1 osteoblasts was higher when exposed to ALG/OTL-s. These data suggest that sodium alginate/otoliths scaffolds are potential biomaterials to be used in bone regeneration applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67995 |
DOI: | 10.1007/s13346-020-00845-x |
ISSN: | 2190-393X |
e-ISSN: | 2190-3948 |
Versão da editora: | https://www.springer.com/journal/13346 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_53887_1.pdf | 3,24 MB | Adobe PDF | Ver/Abrir |