Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/6678
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Cerca, Nuno | - |
dc.contributor.author | Jefferson, Kimberly K. | - |
dc.contributor.author | Maira-Litrán, Tomas | - |
dc.contributor.author | Pier, Danielle B. | - |
dc.contributor.author | Kelly-Quintos, Casie | - |
dc.contributor.author | Goldmann, Donald A. | - |
dc.contributor.author | Azeredo, Joana | - |
dc.contributor.author | Pier, Gerald B. | - |
dc.date.accessioned | 2007-07-09T16:37:27Z | - |
dc.date.available | 2007-07-09T16:37:27Z | - |
dc.date.issued | 2007-07 | - |
dc.identifier.citation | "Infection and Immunity". ISSN 0019-9567. 75:7 (July 2007) 3406-3413. | eng |
dc.identifier.issn | 0019-9567 | eng |
dc.identifier.uri | https://hdl.handle.net/1822/6678 | - |
dc.description.abstract | Poly-N-acetyl-glucosamine (PNAG) is a staphylococcal surface polysaccharide influencing biofilm formation that is also under investigation for its vaccine potential. Antibodies that bind to PNAG with either low (<15%) or high (>90%) levels of acetate are superior at opsonic and protective activity compared with antibodies that bind to PNAG with only high levels (>70%) of acetate. PNAG is synthesized by four proteins encoded within the intercellular adhesin (ica) locus icaADBC. In Staphylococcus epidermidis, icaB encodes a deacetylase needed for the surface retention of PNAG and optimal biofilm formation. In this study, we confirmed that icaB plays a similar role in Staphylococcus aureus and found that an icaB mutant of S. aureus expressed significantly less surface-associated PNAG, was highly susceptible to antibody-independent opsonic killing that could not be enhanced with antibody raised against deacetylated PNAG (dPNAG), and had reduced survival capacity in a murine model of bacteremia. In contrast, an icaB-overexpressing strain produced primarily surface-associated PNAG, was more susceptible to opsonophagocytosis with antibody to dPNAG, and had increased survival in a murine bacteremia model. The highly acetylated secreted PNAG was more effective at blocking opsonic killing mediated by a human monoclonal antibody (mAb) to native PNAG than it was at blocking killing mediated by a human mAb to dPNAG, which by itself was a more effective opsonin. Retention of dPNAG on the surface of S. aureus is key to increased survival during bacteremia and also provides a molecular mechanism explaining the superior opsonic and protective activity of antibody to dPNAG. | eng |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT Portuguese Fulbright Commission; NIH. | eng |
dc.language.iso | eng | eng |
dc.publisher | American Society for Microbiology (ASM) | eng |
dc.rights | openAccess | eng |
dc.title | Molecular basis for preferential protective efficacy of antibodies directed to the poorly acetylated form of staphylococcal poly-N-acetyl-β-(1-6)-glucosamine | eng |
dc.type | article | por |
dc.peerreviewed | yes | eng |
sdum.number | 7 | eng |
sdum.pagination | 3406–3413 | eng |
sdum.publicationstatus | published | eng |
sdum.volume | 75 | eng |
oaire.citationStartPage | 3406 | por |
oaire.citationEndPage | 3413 | por |
oaire.citationIssue | 7 | por |
oaire.citationVolume | 75 | por |
dc.identifier.doi | 10.1128/IAI.00078-07 | por |
dc.identifier.pmid | 17470540 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Infection and Immunity | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Cerca_I&I-07-07[2].pdf | 248,61 kB | Adobe PDF | Ver/Abrir |