Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/65800
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Guimarães, C. F. | por |
dc.contributor.author | Gasperini, L. | por |
dc.contributor.author | Ribeiro, R. S. | por |
dc.contributor.author | Carvalho, A. F. | por |
dc.contributor.author | Marques, A. P. | por |
dc.contributor.author | Reis, R. L. | por |
dc.date.accessioned | 2020-06-30T09:27:19Z | - |
dc.date.available | 2021-07-01T06:00:20Z | - |
dc.date.issued | 2020-06 | - |
dc.date.submitted | 2020-06 | - |
dc.identifier.citation | Guimarães C. F., Gasperini L., Ribeiro R. S., Carvalho A. F., Marques A. P., Reis R. L. High-Throughput Fabrication of Cell-Laden 3D Biomaterial Gradients, Materials Horizons, doi:10.1039/D0MH00818D, 2020 | por |
dc.identifier.issn | 2051-6355 | por |
dc.identifier.uri | https://hdl.handle.net/1822/65800 | - |
dc.description.abstract | High-throughput strategies for optimizing biomaterials to direct cellular behaviour are a fundamental need for propelling tissue engineering and regenerative medicine. In 2D, biomaterialâ s gradients have proven to be powerful platforms for simultaneously screening several surface conditions. However, their translation to 3D is yet limited to 1) exploiting light-based crosslinking and 2) non-sequential, single-gradient production. We built a microfluidic platform that allows distinct hydrogel precursors, as fluids, to be gradually mixed and crosslinked into 3D gradient fibres. Herein, we report how this system can be used for the sequential fabrication of independent cell-laden libraries with gradients of polymer concentration, non-adhesive/adhesive materials and both ionic and light crosslinking mechanisms. Automated image analysis of hundreds of single-cell events as a function of position yielded trends and pinpointed best-fit conditions based on cell shape, adhesion, proliferation and triggering of stromal/stem cell differentiation. We deliver a simple, versatile, and complete approach towards fully high-throughput 3D gradient fabrication for cell/material screening and optimization. | por |
dc.description.sponsorship | The authors acknowledge financial support from the European Research Council, grant ERC-2012-ADG 20120216-321266 (project ComplexiTE). C.F.G. acknowledges scholarship PD/BD/135253/2017 from Fundação para a Ciência e Tecnologia (FCT). Doctor Dillip K. Biship is kindly acknowledged for the help with confocal microscopy. | por |
dc.language.iso | eng | por |
dc.publisher | Royal Society of Chemistry | por |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/321266/EU | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBD%2F135253%2F2017/PT | por |
dc.rights | openAccess | por |
dc.subject | 3D Gradients | por |
dc.subject | Cell-Material Interaction | por |
dc.subject | High-throughput screening | por |
dc.subject | Microfluidics | por |
dc.title | High-throughput fabrication of cell-laden 3D biomaterial gradients | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://doi.org/10.1039/D0MH00818D | por |
dc.comments | http://3bs.uminho.pt/node/20308 | por |
oaire.citationStartPage | 2414 | por |
oaire.citationEndPage | 2421 | por |
oaire.citationIssue | 9 | por |
oaire.citationVolume | 7 | por |
dc.date.updated | 2020-06-30T08:39:07Z | - |
dc.identifier.doi | 10.1039/D0MH00818D | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Materials Horizons | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20308-Paper_Gradients_3Bs.pdf | 2,26 MB | Adobe PDF | Ver/Abrir |