Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/65787

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dc.contributor.authorKurnik, Isabelle S.por
dc.contributor.authorNoronha, Mariana A.por
dc.contributor.authorCâmara, Mayra C. C.por
dc.contributor.authorMazzola, Priscila G.por
dc.contributor.authorVicente, A. A.por
dc.contributor.authorPereira, Jorge F. B.por
dc.contributor.authorLopes, André M.por
dc.date.accessioned2020-06-28T16:12:54Z-
dc.date.available2020-06-28T16:12:54Z-
dc.date.issued2020-11-
dc.identifier.citationKurnik, Isabelle S.; Noronha, Mariana A.; Câmara, Mayra C. C.; Mazzola, Priscila G.; Vicente, António A.; Pereira, Jorge F. B.; Lopes, André M., Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids. Separation and Purification Technology, 250(117262), 2020por
dc.identifier.issn1383-5866por
dc.identifier.urihttp://hdl.handle.net/1822/65787-
dc.description.abstractNovel aqueous two-phase micellar systems (ATPMS) composed of Pluronic F68, a triblock amphiphilic copolymer, and cholinium-based ionic liquids (ILs) were formulated and applied for separation/purification of curcumin (CCM). CCM stability in the presence of ATPMS components was also evaluated. CCM is stable up to 24 h in copolymer (1.0 10.0 wt%) and ILs (0.1 3.0 M) aqueous solutions. Very mild phase separation conditions (close to room temperature) were achieved by adding cholinium ILs to the Pluronic F68 + McIlvaine buffer at pH 6.0 solution. The decrease of cloud-point temperature is dependent on the relative hydrophobicity of IL anion, [Hex] > [But] > [Prop] > [Ac] > Cl. ATPMS composed of more hydrophobic ILs ([Ch][Hex] > [Ch][But] > [Ch][Prop]) are most efficient in the partition of commercial CCM into polymeric micelles-rich phase. The best ATPMS (0.70 M [Ch][But] and 0.60 M [Ch][Hex]-based ATPMS) were then used to purify CCM from a crude extract of Curcuma longa L. Both systems were very selective to separate CCM from protein-based contaminants (selectivity values 25; purification yields 12-fold). Pluronic F68-based ATPMS are promising for selective separation of hydrophobic biomolecules by using cholinium-based ILs as adjuvants to adjust phase separation temperatures and biomolecules partition.por
dc.description.sponsorshipThis study was funded by the Coordination for Higher Level Graduate Improvements (CAPES/Brazil, finance code 001), National Council for Scientific and Technological Development (CNPq/Brazil) and the State of São Paulo Research Foundation (FAPESP/Brazil, processes #2014/16424-7, #2017/10789-1, #2018/10799-0, #2018/05111-9; #2019/05624-9, and #2019/08549-8). A.M. Lopes and J.F.B. Pereira are grateful for the language revision of native speaker H.S. Pacheco Neto.por
dc.language.isoengpor
dc.publisherElsevierpor
dc.rightsopenAccesspor
dc.subjectcurcumin (CCM)por
dc.subjectseparationpor
dc.subjectPluronic F68por
dc.subjectpolymeric micelles (PMs)por
dc.subjectaqueous two-phase micellar systems (ATPMS)por
dc.subjectionic liquids (ILs)por
dc.titleSeparation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquidspor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttps://www.journals.elsevier.com/separation-and-purification-technologypor
dc.commentsCEB53771por
oaire.citationVolume250por
dc.date.updated2020-06-28T11:33:18Z-
dc.identifier.essn1383-5866por
dc.identifier.doi10.1016/j.seppur.2020.117262por
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersion-
dc.subject.wosScience & Technologypor
sdum.journalSeparation and Purification Technologypor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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