Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/65787
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Campo DC | Valor | Idioma |
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dc.contributor.author | Kurnik, Isabelle S. | por |
dc.contributor.author | Noronha, Mariana A. | por |
dc.contributor.author | Câmara, Mayra C. C. | por |
dc.contributor.author | Mazzola, Priscila G. | por |
dc.contributor.author | Vicente, A. A. | por |
dc.contributor.author | Pereira, Jorge F. B. | por |
dc.contributor.author | Lopes, André M. | por |
dc.date.accessioned | 2020-06-28T16:12:54Z | - |
dc.date.available | 2020-06-28T16:12:54Z | - |
dc.date.issued | 2020-11 | - |
dc.identifier.citation | Kurnik, Isabelle S.; Noronha, Mariana A.; Câmara, Mayra C. C.; Mazzola, Priscila G.; Vicente, António A.; Pereira, Jorge F. B.; Lopes, André M., Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids. Separation and Purification Technology, 250(117262), 2020 | por |
dc.identifier.issn | 1383-5866 | por |
dc.identifier.uri | https://hdl.handle.net/1822/65787 | - |
dc.description.abstract | Novel aqueous two-phase micellar systems (ATPMS) composed of Pluronic F68, a triblock amphiphilic copolymer, and cholinium-based ionic liquids (ILs) were formulated and applied for separation/purification of curcumin (CCM). CCM stability in the presence of ATPMS components was also evaluated. CCM is stable up to 24 h in copolymer (1.0 10.0 wt%) and ILs (0.1 3.0 M) aqueous solutions. Very mild phase separation conditions (close to room temperature) were achieved by adding cholinium ILs to the Pluronic F68 + McIlvaine buffer at pH 6.0 solution. The decrease of cloud-point temperature is dependent on the relative hydrophobicity of IL anion, [Hex] > [But] > [Prop] > [Ac] > Cl. ATPMS composed of more hydrophobic ILs ([Ch][Hex] > [Ch][But] > [Ch][Prop]) are most efficient in the partition of commercial CCM into polymeric micelles-rich phase. The best ATPMS (0.70 M [Ch][But] and 0.60 M [Ch][Hex]-based ATPMS) were then used to purify CCM from a crude extract of Curcuma longa L. Both systems were very selective to separate CCM from protein-based contaminants (selectivity values 25; purification yields 12-fold). Pluronic F68-based ATPMS are promising for selective separation of hydrophobic biomolecules by using cholinium-based ILs as adjuvants to adjust phase separation temperatures and biomolecules partition. | por |
dc.description.sponsorship | This study was funded by the Coordination for Higher Level Graduate Improvements (CAPES/Brazil, finance code 001), National Council for Scientific and Technological Development (CNPq/Brazil) and the State of São Paulo Research Foundation (FAPESP/Brazil, processes #2014/16424-7, #2017/10789-1, #2018/10799-0, #2018/05111-9; #2019/05624-9, and #2019/08549-8). A.M. Lopes and J.F.B. Pereira are grateful for the language revision of native speaker H.S. Pacheco Neto. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.rights | openAccess | por |
dc.subject | curcumin (CCM) | por |
dc.subject | separation | por |
dc.subject | Pluronic F68 | por |
dc.subject | polymeric micelles (PMs) | por |
dc.subject | aqueous two-phase micellar systems (ATPMS) | por |
dc.subject | ionic liquids (ILs) | por |
dc.title | Separation and purification of curcumin using novel aqueous two-phase micellar systems composed of amphiphilic copolymer and cholinium ionic liquids | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.journals.elsevier.com/separation-and-purification-technology | por |
dc.comments | CEB53771 | por |
oaire.citationVolume | 250 | por |
dc.date.updated | 2020-06-28T11:33:18Z | - |
dc.identifier.eissn | 1383-5866 | por |
dc.identifier.doi | 10.1016/j.seppur.2020.117262 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Separation and Purification Technology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53771_1.pdf | 6,42 MB | Adobe PDF | Ver/Abrir |