Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/65778
Título: | Portable device for optical quantification of hemozoin in diluted blood samples |
Autor(es): | Catarino, Susana Oliveira Félix, Paulo Sousa, Paulo J. Pinto, Vânia Veiga, Maria Isabel Minas, Graça |
Palavras-chave: | Light emitting diodes Diseases Blood Biomedical optical imaging Optical devices Photodiodes Power supplies Hemozoin Malaria Medical device Point-of-care Sensor |
Data: | 1-Fev-2020 |
Editora: | Institute of Electrical and Electronics Engineers (IEEE) |
Revista: | IEEE Transactions on Biomedical Engineering |
Citação: | Catarino, S. O., Félix, P., Sousa, P. J., et. al. (2019). Portable Device for Optical Quantification of Hemozoin in Diluted Blood Samples. IEEE Transactions on Biomedical Engineering, 67(2), 365-371 |
Resumo(s): | Objective: This paper focuses on a novel and portable device prototype with optical detectors to quickly and efficiently detect hemozoin (Hz) in blood, aiming at malaria diagnostics. Methods: Taking advantage of the particular features of malaria parasite in infected blood, particularly the Hz formation, the main innovation described is a portable device for the optical quantification of parasitic Hz in blood, through optical absorbance spectrophotometry. This device comprises detection chambers for fluidic samples, an optical emission and detection system, and a power supply system to provide autonomy. The working principle is based on colorimetric detection, by absorbance, at six specific wavelengths. A detection algorithm relates the absorbance values at all wavelengths to quantify the Hz concentration, thus working as a biomarker of malaria presence and stage. Results: Under the tested conditions, e.g., in fluidic samples containing synthetic Hz, hemoglobin, and diluted whole blood, the device detected Hz above 1& x00A0;& x03BC;g& x002F;mL concentrations with 100& x0025; sensitivity and 96.3& x0025; specificity. Conclusion: This paper features an autonomous, portable, 1-min analysis time, and low-cost per test device, without the need for samples, centrifugation, allowing the use of whole blood. Significance: The presented device is a step ahead for meeting the growing clinical demands for reliable, rapid, portable, and quantitative malaria diagnosis. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/65778 |
DOI: | 10.1109/TBME.2019.2913454 |
ISSN: | 0018-9294 |
Versão da editora: | https://ieeexplore.ieee.org/abstract/document/8700235 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
Aparece nas coleções: | CMEMS - Artigos em revistas internacionais/Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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08700235 Final publication.pdf Acesso restrito! | 3,38 MB | Adobe PDF | Ver/Abrir |