Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/65633
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Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, Amélia M. | por |
dc.contributor.author | Martins-Gomes, Carlos | por |
dc.contributor.author | Souto, Eliana B. | por |
dc.contributor.author | Schäfer, Judith | por |
dc.contributor.author | Santos, João A. | por |
dc.contributor.author | Bunzel, Mirko | por |
dc.contributor.author | Nunes, Fernando M. | por |
dc.date.accessioned | 2020-06-15T15:05:25Z | - |
dc.date.available | 2020-06-15T15:05:25Z | - |
dc.date.issued | 2020-06-03 | - |
dc.identifier.citation | Silva, Amélia M.; Martins-Gomes, Carlos; Souto, Eliana; Schäfer, Judith; Santos, João A.; Bunzel, Mirko; Nunes, Fernando M., Thymus zygis subsp. zygis an endemic portuguese plant: phytochemical profiling, antioxidant, anti-proliferative and anti-inflammatory activities. Antioxidants, 9(6), 482, 2020 | por |
dc.identifier.uri | https://hdl.handle.net/1822/65633 | - |
dc.description.abstract | Thymus zygis subsp. zygis is an endemic Portuguese plant belonging to the Thymus zygis species. Although T. zygis is commonly used as a condiment and as a medicinal herb, a detailed description of the polyphenol composition of hydroethanolic (HE) and aqueous decoction (AD) extracts is not available. In this work, we describe for the first time a detailed phenolic composition of Thymus zygis subsp. zygis HE and AD extracts, together with their antioxidant, anti-proliferative and anti-inflammatory activities. Unlike other Thymus species, T. zygis subsp. zygis extracts contain higher amounts of luteolin-(?)-O-hexoside. However, the major phenolic compound is rosmarinic acid, and high amounts of salvianolic acids K and I were also detected. T. zygis subsp. zygis extracts exhibited significant scavenging activity of ABTS+, hydroxyl (•OH), and nitric oxide (NO) radicals. Regarding the anti-proliferative/cytotoxic effect, tested against Caco-2 and HepG2 cells, the AD extract only slightly reduced cell viability at higher concentrations (IC50 > 600 µg/mL, 48 h exposure), denoting very low toxicity, while the HE extract showed a high anti-proliferative effect, especially at 48 h exposure (IC50 of 85.01 ± 15.10 μg/mL and 82.19 ± 2.46 μg/mL, for Caco-2 and HepG2, respectively). At non-cytotoxic concentrations, both extracts reduced the nitric oxide (NO) release by lipopolysaccharide (LPS)-stimulated RAW 264.7 cells (at 50 μg/mL, HE and AD extracts inhibited NO release in ~89% and 48%, respectively). In conclusion, the results highlight the non-toxic effect of aqueous extracts, both resembling the consumption of antioxidants in foodstuff or in functional food. Furthermore, the HE extract of T. zygis subsp. zygis is a source of promising molecules with antioxidant, anti-inflammatory and anticancer activities, highlighting its potential as a source of bioactive ingredients for nutraceutical and pharmaceutical industries. | por |
dc.description.sponsorship | This work was supported by the INTERACT project–“Integrative Research in Environment, Agro-Chains and Technology”, no. NORTE-01-0145- FEDER-000017, in its line of research entitled ISAC, co-financed by the European Regional Development Fund (ERDF) through NORTE 2020 (North Regional Operational Program 2014/2020). By funds from the Portuguese Science and Technology Foundation, Ministry of Science and Education (FCT/MEC) through national funds, under the projects UIDB/04033/2020 (CITAB), UIDB/00616/2020 (CQ-VR) and UIDB/04469/2020 (CEB). FCT is also acknowledged for the grant to C.M.G. (SFRH/BD/145855/2019). | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.relation | UIDB/04033/2020 | por |
dc.relation | UIDB/00616/2020 | por |
dc.relation | UIDB/04469/2020 | por |
dc.relation | SFRH/BD/145855/2019 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | Thymus zygis subsp. zygis | por |
dc.subject | phenolic profiling | por |
dc.subject | aqueous decoction | por |
dc.subject | hydroethanolic extract | por |
dc.subject | luteolin-O-hexoside | por |
dc.subject | anti-proliferative activity | por |
dc.subject | radical scavenging activity | por |
dc.subject | antioxidant | por |
dc.subject | anti-inflammatory activity | por |
dc.subject | Thymus zygis subsp | por |
dc.subject | Zygis | por |
dc.title | Thymus zygis subsp. zygis an endemic portuguese plant: phytochemical profiling, antioxidant, anti-proliferative and anti-inflammatory activities | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/journal/antioxidants | por |
dc.comments | CEB53753 | por |
oaire.citationIssue | 6 | por |
oaire.citationVolume | 9 | por |
dc.date.updated | 2020-06-07T16:33:50Z | - |
dc.identifier.eissn | 2076-3921 | por |
dc.identifier.doi | 10.3390/antiox9060482 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Antioxidants | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53753_1.pdf | 1,89 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons