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dc.contributor.authorTavares, Tânia Daniela Eugéniopor
dc.contributor.authorAntunes, Joana Isabel Costapor
dc.contributor.authorPadrão, Jorgepor
dc.contributor.authorRibeiro, Ana Isabel Ferreirapor
dc.contributor.authorZille, Andreapor
dc.contributor.authorAmorim, M. T. Pessoa depor
dc.contributor.authorFerreira, Fernandopor
dc.contributor.authorFelgueiras, Helena Pradopor
dc.date.accessioned2020-06-09T14:53:39Z-
dc.date.available2020-06-09T14:53:39Z-
dc.date.issued2020-
dc.identifier.issn2079-6382por
dc.identifier.urihttps://hdl.handle.net/1822/65603-
dc.description.abstractThe increased resistance of bacteria against conventional pharmaceutical solutions, the antibiotics, has raised serious health concerns. This has stimulated interest in the development of bio-based therapeutics with limited resistance, namely, essential oils (EOs) or antimicrobial peptides (AMPs). This study envisaged the evaluation of the antimicrobial efficacy of selected biomolecules, namely LL37, pexiganan, tea tree oil (TTO), cinnamon leaf oil (CLO) and niaouli oil (NO), against four bacteria commonly associated to nosocomial infections: Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa. The antibiotic vancomycin and silver nanoparticles (AgNPs) were used as control compounds for comparison purposes. The biomolecules were initially screened for their antibacterial efficacy using the agar-diffusion test, followed by the determination of minimal inhibitory concentrations (MICs), kill-time kinetics and the evaluation of the cell morphology upon 24 h exposure. All agents were effective against the selected bacteria. Interestingly, the AgNPs required a higher concentration (4000‐1250 µg/mL) to induce the same effects as the AMPs (500‐7.8 µg/mL) or EOs (365.2‐19.7 µg/mL). Pexiganan and CLO were the most effective biomolecules, requiring lower concentrations to kill both Gram-positive and Gram-negative bacteria (62.5‐7.8 µg/mL and 39.3‐19.7 µg/mL, respectively), within a short period of time (averaging 2 h 15 min for all bacteria). Most biomolecules apparently disrupted the bacteria membrane stability due to the observed cell morphology deformation and by effecting on the intracellular space. AMPs were observed to induce morphological deformations and cellular content release, while EOs were seen to split and completely envelope bacteria. Data unraveled more of the potential of these new biomolecules as replacements for the conventional antibiotics and allowed us to take a step forward in the understanding of their mechanisms of action against infection-related bacteria.por
dc.description.sponsorshipThis research received funding from the Portuguese Foundation for Science and Technology (FCT) under the scope of the projects PTDC/CTM‐TEX/28074/2017 (POCI‐01‐0145‐FEDER‐028074), PTDC/CTM‐ TEX/28295/2017 and UID/CTM/00264/2020.por
dc.language.isoengpor
dc.publisherMDPIpor
dc.relationPTDC/CTM‐TEX/28074/2017por
dc.relationPTDC/CTM‐ TEX/28295/2017por
dc.relationUID/CTM/00264/2020por
dc.rightsopenAccesspor
dc.subjectAntimicrobial peptidespor
dc.subjectEssential oilspor
dc.subjectMinimum inhibitory concentrationpor
dc.subjectBactericidalpor
dc.subjectNosocomialpor
dc.titleActivity of specialized biomolecules against Gram-positive and Gram-negative bacteriapor
dc.typearticlepor
dc.peerreviewedyespor
dc.relation.publisherversionhttps://www.mdpi.com/journal/antibioticspor
oaire.citationStartPage1por
oaire.citationEndPage16por
oaire.citationIssue6por
oaire.citationVolume9por
dc.identifier.eissn2079-6382por
dc.identifier.doi10.3390/antibiotics9060314por
dc.subject.fosEngenharia e Tecnologia::Engenharia dos Materiaispor
dc.subject.wosScience & Technologypor
sdum.journalAntibioticspor
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