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https://hdl.handle.net/1822/64413
Título: | Development of dextrin-amphotericin B formulations for the treatment of Leishmaniasis |
Autor(es): | Carvalho, Ricardo Emanuel Silva Fidalgo, Joaquim Melo, K. R Queiroz, M. F. Leal, Ana Salomé Barbosa Rocha, H. A. Cruz, T. Parpot, Pier Tomás, A. M. Gama, F. M. |
Palavras-chave: | Dextrin Amphotericin B Leishmaniasis |
Data: | 15-Jun-2020 |
Editora: | Elsevier 1 |
Revista: | International Journal of Biological Macromolecules |
Citação: | Silva-Carvalho, Ricardo; Fidalgo, J.; Melo, K. R.; Queiroz, M. F.; Leal, S.; Rocha, H. A.; Cruz, T.; Parpot, Pier; Tomás, A. M.; Gama, F. M., Development of dextrin-amphotericin B formulations for the treatment of Leishmaniasis. International Journal of Biological Macromolecules, 153, 276-288, 2020 |
Resumo(s): | The most effective medicines available for the treatment of leishmaniasis, a life-threatening disease, exhibit serious toxicological issues. To achieve better therapeutic efficiency while decreasing toxicity associated with amphotericin B (AmB), water-soluble dextrin-AmB (Dex-AmB) formulations were developed. Self-assembled nanocomplexes were formed by dissolving Dex and AmB in alkaline borate buffer, followed by dialysis and either freeze-drying (FD) or nano spray-drying (SD), yielding water dispersible particles with a diameter of 214nm and 347nm, respectively. The very simple production process allowed the formation of amorphous inclusion complexes containing 14% of AmB in the form of monomers and water-soluble aggregates. Nanocomplexes were effective against parasites in axenic culture (IC50 of 0.056 and 0.096M for L. amazonensis and 0.030 and 0.044M for L. infantum, respectively for Dex-AmB FD and Dex-AmB SD) and in decreasing the intramacrophagic infection with L. infantum (IC50 of 0.017 and 0.023M, respectively for Dex-AmB FD and Dex-AmB SD). Also, the formulations were able to significantly reduce the cytotoxicity of AmB. Overall, this study demonstrates the suitability of dextrin as an AmB carrier and the facile and inexpensive development of a delivery system for the treatment of leishmaniasis. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/64413 |
DOI: | 10.1016/j.ijbiomac.2020.03.019 |
ISSN: | 0141-8130 |
e-ISSN: | 1879-0003 |
Versão da editora: | http://www.elsevier.com/locate/issn/01418130 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53547_1.pdf | 4,02 MB | Adobe PDF | Ver/Abrir |