Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/64376

TitleThree-dimensional self-assembling nanofiber matrix rejuvenates aged/degenerative human tendon stem/progenitor cells
Author(s)Yin, Heyong
Strunz, Franziska
Yan, Zexing
Lu, Jiaju
Brochhausen, Christoph
Kiderlen, Stefanie
Clausen-Schaumann, Hauke
Wang, Xiumei
Gomes, M. J. M.
Alt, Volker
et al.
Keywords3D microenvironment
Cell
Cell aging
Hydrogel
Rejuvenation
Stem/progenitor
Stem/progenitor cell
Issue dateJan-2020
PublisherElsevier
JournalBiomaterials
CitationYina H., Strunza F., Yana Z., Luc J., Brochhausend C., Kiderlene S., Clausen-Schaum H., Wang X., Gomes M. E., Alta V., Docheva D. Three-dimensional self-assembling nanofiber matrix rejuvenates aged/degenerative human tendon stem/progenitor cells, Biomaterials, Vol. 236, doi:10.1016/j.biomaterials.2020.119802, 2020
Abstract(s)The poor healing capacity of tendons is known to worsen in the elderly. During tendon aging and degeneration, endogenous human tendon stem/progenitor cells (hTSPCs) experience profound pathological changes. Here, we explored a rejuvenation strategy for hTSPCs derived from aged/degenerated Achilles tendons (A-TSPCs) by providing three-dimensional (3D) nanofiber hydrogels and comparing them to young/healthy TSPCs (Y-TSPCs). RADA peptide hydrogel has a self-assembling ability, forms a nanofibrous 3D niche and can be further functionalized by adding RGD motifs. Cell survival, apoptosis, and proliferation assays demonstrated that RADA and RADA/RGD hydrogels support A-TSPCs in a comparable manner to Y-TSPCs. Moreover, they rejuvenated ATSPCs to a phenotype similar to that of Y-TSPCs, as evidenced by restored cell morphology and cytoskeletal architecture. Transmission electron, confocal laser scanning and atomic force microscopies demonstrated comparable ultrastructure, surface roughness and elastic modulus of A- and Y-TSPC-loaded hydrogels. Lastly, quantitative PCR revealed similar expression profiles, as well a significant upregulation of genes related to tenogenesis and multipotency. Taken together, the RADA-based hydrogels exert a rejuvenating effect by recapitulating in vitro specific features of the natural microenvironment of human TSPCs, which strongly indicates their potential to direct cell behaviour and overcome the challenge of cell aging and degeneration in tendon repair.
TypeArticle
URIhttp://hdl.handle.net/1822/64376
DOI10.1016/j.biomaterials.2020.119802
ISSN0142-9612
e-ISSN1878-5905
Publisher versionhttps://www.sciencedirect.com/science/article/pii/S014296122030048X
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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