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https://hdl.handle.net/1822/64376
Título: | Three-dimensional self-assembling nanofiber matrix rejuvenates aged/degenerative human tendon stem/progenitor cells |
Autor(es): | Yin, Heyong Strunz, Franziska Yan, Zexing Lu, Jiaju Brochhausen, Christoph Kiderlen, Stefanie Clausen-Schaumann, Hauke Wang, Xiumei Gomes, Manuela E. Alt, Volker Docheva, Denitsa |
Palavras-chave: | 3D microenvironment Cell Cell aging Hydrogel Rejuvenation Stem/progenitor Stem/progenitor cell |
Data: | Jan-2020 |
Editora: | Elsevier 1 |
Revista: | Biomaterials |
Citação: | Yina H., Strunza F., Yana Z., Luc J., Brochhausend C., Kiderlene S., Clausen-Schaum H., Wang X., Gomes M. E., Alta V., Docheva D. Three-dimensional self-assembling nanofiber matrix rejuvenates aged/degenerative human tendon stem/progenitor cells, Biomaterials, Vol. 236, doi:10.1016/j.biomaterials.2020.119802, 2020 |
Resumo(s): | The poor healing capacity of tendons is known to worsen in the elderly. During tendon aging and degeneration, endogenous human tendon stem/progenitor cells (hTSPCs) experience profound pathological changes. Here, we explored a rejuvenation strategy for hTSPCs derived from aged/degenerated Achilles tendons (A-TSPCs) by providing three-dimensional (3D) nanofiber hydrogels and comparing them to young/healthy TSPCs (Y-TSPCs). RADA peptide hydrogel has a self-assembling ability, forms a nanofibrous 3D niche and can be further functionalized by adding RGD motifs. Cell survival, apoptosis, and proliferation assays demonstrated that RADA and RADA/RGD hydrogels support A-TSPCs in a comparable manner to Y-TSPCs. Moreover, they rejuvenated ATSPCs to a phenotype similar to that of Y-TSPCs, as evidenced by restored cell morphology and cytoskeletal architecture. Transmission electron, confocal laser scanning and atomic force microscopies demonstrated comparable ultrastructure, surface roughness and elastic modulus of A- and Y-TSPC-loaded hydrogels. Lastly, quantitative PCR revealed similar expression profiles, as well a significant upregulation of genes related to tenogenesis and multipotency. Taken together, the RADA-based hydrogels exert a rejuvenating effect by recapitulating in vitro specific features of the natural microenvironment of human TSPCs, which strongly indicates their potential to direct cell behaviour and overcome the challenge of cell aging and degeneration in tendon repair. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/64376 |
DOI: | 10.1016/j.biomaterials.2020.119802 |
ISSN: | 0142-9612 |
e-ISSN: | 1878-5905 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S014296122030048X |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20266-DenitsaBiomaterials2020.pdf | 8,17 MB | Adobe PDF | Ver/Abrir |