Please use this identifier to cite or link to this item:

TitleThree-dimensional self-assembling nanofiber matrix rejuvenates aged/degenerative human tendon stem/progenitor cells
Author(s)Yin, Heyong
Strunz, Franziska
Yan, Zexing
Lu, Jiaju
Brochhausen, Christoph
Kiderlen, Stefanie
Clausen-Schaumann, Hauke
Wang, Xiumei
Gomes, Manuela E.
Alt, Volker
Docheva, Denitsa
Keywords3D microenvironment
Cell aging
Stem/progenitor cell
Issue dateJan-2020
CitationYina H., Strunza F., Yana Z., Luc J., Brochhausend C., Kiderlene S., Clausen-Schaum H., Wang X., Gomes M. E., Alta V., Docheva D. Three-dimensional self-assembling nanofiber matrix rejuvenates aged/degenerative human tendon stem/progenitor cells, Biomaterials, Vol. 236, doi:10.1016/j.biomaterials.2020.119802, 2020
Abstract(s)The poor healing capacity of tendons is known to worsen in the elderly. During tendon aging and degeneration, endogenous human tendon stem/progenitor cells (hTSPCs) experience profound pathological changes. Here, we explored a rejuvenation strategy for hTSPCs derived from aged/degenerated Achilles tendons (A-TSPCs) by providing three-dimensional (3D) nanofiber hydrogels and comparing them to young/healthy TSPCs (Y-TSPCs). RADA peptide hydrogel has a self-assembling ability, forms a nanofibrous 3D niche and can be further functionalized by adding RGD motifs. Cell survival, apoptosis, and proliferation assays demonstrated that RADA and RADA/RGD hydrogels support A-TSPCs in a comparable manner to Y-TSPCs. Moreover, they rejuvenated ATSPCs to a phenotype similar to that of Y-TSPCs, as evidenced by restored cell morphology and cytoskeletal architecture. Transmission electron, confocal laser scanning and atomic force microscopies demonstrated comparable ultrastructure, surface roughness and elastic modulus of A- and Y-TSPC-loaded hydrogels. Lastly, quantitative PCR revealed similar expression profiles, as well a significant upregulation of genes related to tenogenesis and multipotency. Taken together, the RADA-based hydrogels exert a rejuvenating effect by recapitulating in vitro specific features of the natural microenvironment of human TSPCs, which strongly indicates their potential to direct cell behaviour and overcome the challenge of cell aging and degeneration in tendon repair.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
20266-DenitsaBiomaterials2020.pdf8,17 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID