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https://hdl.handle.net/1822/64263
Título: | Effect of sub-lethal chemical disinfection on the biofilm forming ability, resistance to antibiotics and expression of virulence genes of Salmonella Enteritidis biofilm-surviving cells |
Autor(es): | Romeu, M. Rodrigues, Diana Alexandra Ferreira Azeredo, Joana |
Palavras-chave: | Foodborne pathogens cross-resistance Salmonella Enteritidis chemical disinfectants biofilm virulence |
Data: | 30-Jan-2020 |
Editora: | Taylor and Francis |
Revista: | Biofouling |
Citação: | Romeu, M.; Rodrigues, Diana; Azeredo, Joana, Effect of sub-lethal chemical disinfection on the biofilm forming ability, resistance to antibiotics and expression of virulence genes of Salmonella Enteritidis biofilm-surviving cells. Biofouling, 36(1), 101-112, 2020 |
Resumo(s): | Although disinfection procedures are widely implemented in food environments, bacteria can survive and present increased virulence/resistance. Since little is known about these phenomena regarding biofilms, this study aimed to investigate the effect of chemical disinfection on biofilm-derived cells of Salmonella Enteritidis. Using a reference strain (NCTC 13349) and a food isolate (350), biofilm susceptibility to benzalkonium chloride (BAC), sodium hypochlorite (SH) and hydrogen peroxide (HP) was evaluated and biofilms were exposed to sub-lethal concentrations of each disinfectant. Biofilm-derived cells were characterized for their biofilm forming ability, antibiotic resistance and expression of virulence-associated genes. Except for a few instances, disinfectant exposure did not alter antibiotic susceptibility. However, SH and HP exposure enhanced the biofilm forming ability of Salmonella Enteritidis NCTC 13349. After BAC and HP exposure, biofilm-derived cells presented a down-regulation of rpoS. Exposure to BAC also revealed an up-regulation of invA, avrA and csgD on Salmonella Enteritidis NCTC 13349. The results obtained suggest that biofilm-derived cells that survive disinfection may represent an increased health risk. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/64263 |
DOI: | 10.1080/08927014.2020.1719077 |
ISSN: | 0892-7014 |
e-ISSN: | 1029-2454 |
Versão da editora: | http://www.tandfonline.com/action/journalInformation?journalCode=gbif20 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53444_1.pdf | 1,16 MB | Adobe PDF | Ver/Abrir |