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TitlePhospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration
Author(s)Laomeephol, Chavee
Guedes, Marta Cristina Alves Moreira
Ferreira, Helena Susana Costa Machado
Reis, R. L.
Kanokpanont, Sorada
Damrongsakkul, Siriporn
Neves, N. M.
KeywordsCell encapsulation
Gelation time
Silk Fibroin
Issue date2020
JournalJournal of Tissue Engineering and Regenerative Medicine
CitationLaomeephol C., Guedes M., Ferreira H., Reis R. L., Kanokpanont S., Damrongsakkul S., Neves N. M. Phospholipid‐induced silk fibroin hydrogels and their potential as cell carriers for tissue regeneration, Journal Of Tissue Engineering And Regenerative Medicine, pp. 1-13, doi:10.1002/term.2982, 2020
Abstract(s)Silk fibroin (SF) hydrogels can be obtained via self‐assembly, but this process takes several days or weeks, being unfeasible to produce cell carrier hydrogels. In this work, a phospholipid, namely, 1,2‐dimyristoyl‐sn‐glycero‐3‐phospho‐(1′‐rac‐glycerol) sodium salt (DMPG), was used to induce and accelerate the gelation process of SF solutions. Due to the amphipathic nature and negative charge of DMPG, electrostatic and hydrophobic interactions between the phospholipids and SF chains will occur, inducing the structural transition of SF chains to the beta sheet and consequently a rapid gel formation is observed (less than 50 min). Moreover, the gelation time can be controlled by varying the lipid concentration. To assess the potential of the hydrogels as cell carriers, several mammalian cell lines, including L929, NIH/3T3, SaOS‐2, and CaSki, were encapsulated into the hydrogel. The silk‐based hydrogels supported the normal growth of fibroblasts, corroborating their cytocompatibility. Interestingly, an inhibition in the growth of cancer‐derived cell lines was observed. Therefore, DMPG‐induced SF hydrogels can be successfully used as a 3D platform for in situ cell encapsulation, opening promising opportunities in biomedical applications, such as in cell therapies and tissue regeneration.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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