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https://hdl.handle.net/1822/62021
Título: | Synergistic effect of hen egg white lysozyme and lysosomotropic surfactants on cell viability and membrane permeability |
Autor(es): | Tomasz Janek Rodrigues, L. R. Gudiña, Eduardo J. Joanna Burger |
Palavras-chave: | Lysozyme Lysosomotropic substances Surface tension Circular dichroism Isothermal titration calorimetry |
Data: | 2020 |
Editora: | Elsevier 1 |
Revista: | Colloids and Surfaces B: Biointerfaces |
Citação: | Tomasz Janek; Rodrigues, Lígia R.; Gudiña, Eduardo J.; Joanna Burger, Synergistic effect of hen egg white lysozyme and lysosomotropic surfactants on cell viability and membrane permeability. Colloids and Surfaces B- Biointerfaces, 185, 110598-110598, 2020 |
Resumo(s): | The interactions between two types of quaternary ammonium surfactants (N,N,N-trimethyl-2-(dodecanoyloxy)ethaneammonium bromide (DMM-11) and N,N,N-trimethyl-2-(dodecanoyloxy)propaneammonium bromide (DMPM-11)) and hen egg white lysozyme were studied through several techniques, including isothermal titration calorimetry (ITC), circular dichroism (CD) and fluorescence spectroscopy, and surface tension measurement. The average number of surfactants interacting with each molecule of lysozyme was calculated from the biophysical results. Moreover, the CD results showed that the conformation of lysozyme changed in the presence of DMM-11 and DMPM-11. The studies drew a detailed picture on the physicochemical nature of interactions between both surfactants and lysozyme. Both DMM-11 and DMPM-11, with and without lysozyme were studied against three target microorganisms, including Gram-negative (Escherichia coli) and Gram-positive (Enterococcus hirae and Enterococcus faecalis) bacteria. The results revealed a broad spectrum of antibacterial nature of surfactant/lysozyme complexes, as well as their effect on the membrane damage, hence providing the basis to further explore DMM-11 and DMPM-11 combined with lysozyme as possible antibacterial tools. |
Tipo: | Artigo |
Descrição: | Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.colsurfb.2019.110598. |
URI: | https://hdl.handle.net/1822/62021 |
DOI: | 10.1016/j.colsurfb.2019.110598 |
ISSN: | 0927-7765 |
e-ISSN: | 0927-7765 |
Versão da editora: | http://www.journals.elsevier.com/colloids-and-surfaces-b-biointerfaces/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_52183_1.pdf | 2,41 MB | Adobe PDF | Ver/Abrir |