Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61821
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Carneiro, Mariana | por |
dc.contributor.author | Sousa-Castillo, Ana | por |
dc.contributor.author | Correa-Duarte, Miguel A. | por |
dc.contributor.author | Sales, M. G. F. | por |
dc.date.accessioned | 2019-10-21T09:15:23Z | - |
dc.date.issued | 2019-12 | - |
dc.identifier.citation | Carneiro, Mariana; Sousa-Castillo, Ana; Correa-Duarte, Miguel A.; Sales, M. G. F., Dual biorecognition by combining molecularly-imprinted polymer and antibody in SERS detection. Application to carcinoembryonic antigen. Biosensors & Bioelectronics, 146(111761), 2019 | por |
dc.identifier.issn | 0956-5663 | por |
dc.identifier.uri | https://hdl.handle.net/1822/61821 | - |
dc.description | Supplementary data to this article can be found online at https://doi. org/10.1016/j.bios.2019.111761. | por |
dc.description.abstract | This work reports the innovative combination of a molecularly-imprinted polymer (MIP) and a natural antibody for the accurate surface-enhanced Raman spectroscopy (SERS) detection of carcinoembryonic antigen (CEA). The MIP material acted as a pre-concentration scheme for the target protein, while the natural antibody was responsible to signal the presence of CEA on the MIP platform. Gold-based screen-printed electrodes were used as substrate and gallic acid (GA) was used herein for the first time in the assembly of a MIP film, by electropolymerization, in the presence of CEA. This layer was further covered by a second ultra-thin film of electropolymerized benzoic acid (BA), to avoid non-specific binding. The rebinding features of the MIP film were evaluated by electrochemical impedance spectroscopy (EIS) and a linear response was observed from 1 to 1000ng/mL. For a sensitive SERS detection, the MIP film was first incubated in sample containing CEA and next incubated in SERS tag. For the SERS tag, gold nanostars (AuNSs) were employed as metal support, coupled to 4-aminothiophenol (4-ATP) as Raman reporter and to a natural antibody for CEA as recognition element. The overall system showed a sensitive response down to 1.0ng/mL, which was different from the blank signal. Overall, the innovative approach presented herein combines the advantages of using two different targeting elements for CEA. The costs and time of MIP production were substantially low due to selection of electropolymerization approach and the proposal described herein may be extended to other target molecules. | por |
dc.description.sponsorship | Authors acknowledge funding to FEDER/COMPETE 2020 through the project RamSERS (NORTE-01-0247-FEDER-017834, call no. 33/SI/ 2015), to POCTEP through the project IBEROS (0245_IBEROS_1_E), Fundação para a Ciência e Tecnologia, I.P., through the PhD grant reference SFRH/BD/131959/2017, MINECO-Spain CTM2017-84050-R and Xunta de Galicia (IN607A 2018/5 and Acc. 2016–2019). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | SFRH/BD/131959/2017 | por |
dc.rights | restrictedAccess | por |
dc.subject | Biosensor | por |
dc.subject | Surface-enhanced Raman spectroscopy | por |
dc.subject | Molecularly-imprinted polymer | por |
dc.subject | Carcinoembryonic antigen | por |
dc.subject | CEA-Antibody | por |
dc.subject | Gold nanostars | por |
dc.title | Dual biorecognition by combining molecularly-imprinted polymer and antibody in SERS detection. Application to carcinoembryonic antigen | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/biosensors-and-bioelectronics/ | por |
dc.comments | CEB52074 | por |
oaire.citationVolume | 146 | por |
dc.date.updated | 2019-10-21T08:34:24Z | - |
dc.identifier.doi | 10.1016/j.bios.2019.111761 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 31614254 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biosensors & Bioelectronics | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_52074_1.pdf Acesso restrito! | 1,37 MB | Adobe PDF | Ver/Abrir |