Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61691
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Felgueiras, Helena Prado | por |
dc.contributor.author | Teixeira, Marta Albertina | por |
dc.contributor.author | Tavares, Tânia Daniela Eugénio | por |
dc.contributor.author | Homem, Natália Cândido | por |
dc.contributor.author | Zille, Andrea | por |
dc.contributor.author | Amorim, M. T. Pessoa de | por |
dc.date.accessioned | 2019-10-09T13:10:33Z | - |
dc.date.available | 2019-10-09T13:10:33Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 0021-8995 | por |
dc.identifier.uri | https://hdl.handle.net/1822/61691 | - |
dc.description.abstract | Poly(vinyl alcohol)/cellulose acetate (PVA/CA) films were prepared via a new method that combines principles from solvent casting and phase inversion. To guarantee some degree of flexibility, films were produced with a higher percentage of PVA compared to CA, from 90/10 to 50/50. The antimicrobial peptide (AMP) LL37 was then anchored using dopamine as a binding agent. Films were characterized in terms of functional groups, thermal stability, tensile strength, porosity, swelling and degradation (stability in physiological media at different pHs). The antimicrobial performance of LL37 surface-modified films was tested against Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli in dynamic environment and in the presence and absence of an albumin interface. LL37 treated films demonstrated great antibacterial efficacy against the three kinds of bacteria, ≈ 75% inhibition for S. aureus, ≈ 85% for S. epidermidis and ≈ 60% for E. coli, regardless of PVA/CA ratio. Presence of albumin reduced bacteria inhibition in all tested groups, most likely due to the binding of the protein molecules to the antimicrobial agents, reducing the free fraction available for bacterial killing. Films treated with LL37 accelerated clotting time (≈ 10 min) above vancomycin and bare surfaces, demonstrating great capacity to activate the intrinsic coagulation cascade. | por |
dc.description.sponsorship | Portuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTES | por |
dc.language.iso | eng | por |
dc.publisher | Wiley | por |
dc.relation | UID/CTM/00264/2019 | - |
dc.rights | openAccess | por |
dc.subject | Biodegradable films | por |
dc.subject | Peptide LL37 | por |
dc.subject | Antibacterial action | por |
dc.subject | Accelerated clotting | por |
dc.subject | Wound-healing | por |
dc.subject | biocompatibility | por |
dc.subject | biodegradable | por |
dc.subject | bioengineering | por |
dc.subject | biomedical Applications | por |
dc.subject | films | por |
dc.title | Antimicrobial action and clotting time of thin, hydrated poly(Vinyl Alcohol)/cellulose acetate films functionalized with LL37 for prospective wound-healing applications | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
oaire.citationIssue | 18 | por |
oaire.citationVolume | 137 | por |
dc.identifier.doi | 10.1002/app.48626 | por |
dc.subject.fos | Engenharia e Tecnologia::Engenharia dos Materiais | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Applied Polymer Science | por |
oaire.version | AM | por |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2019.10.03_Manuscript PVA-CA black.pdf | 1,32 MB | Adobe PDF | Ver/Abrir |