Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/61691

Full metadata record
DC FieldValueLanguage
dc.contributor.authorFelgueiras, Helena Pradopor
dc.contributor.authorTeixeira, Marta Albertinapor
dc.contributor.authorTavares, Tânia Daniela Eugéniopor
dc.contributor.authorHomem, Natália Cândidopor
dc.contributor.authorZille, Andreapor
dc.contributor.authorAmorim, M. T. Pessoa depor
dc.date.accessioned2019-10-09T13:10:33Z-
dc.date.available2019-10-09T13:10:33Z-
dc.date.issued2019-
dc.identifier.issn0021-8995por
dc.identifier.urihttp://hdl.handle.net/1822/61691-
dc.descriptionPreprint versionpor
dc.description.abstractPoly(vinyl alcohol)/cellulose acetate (PVA/CA) films were prepared via a new method that combines principles from solvent casting and phase inversion. To guarantee some degree of flexibility, films were produced with a higher percentage of PVA compared to CA, from 90/10 to 50/50. The antimicrobial peptide (AMP) LL37 was then anchored using dopamine as a binding agent. Films were characterized in terms of functional groups, thermal stability, tensile strength, porosity, swelling and degradation (stability in physiological media at different pHs). The antimicrobial performance of LL37 surface-modified films was tested against Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli in dynamic environment and in the presence and absence of an albumin interface. LL37 treated films demonstrated great antibacterial efficacy against the three kinds of bacteria, ≈ 75% inhibition for S. aureus, ≈ 85% for S. epidermidis and ≈ 60% for E. coli, regardless of PVA/CA ratio. Presence of albumin reduced bacteria inhibition in all tested groups, most likely due to the binding of the protein molecules to the antimicrobial agents, reducing the free fraction available for bacterial killing. Films treated with LL37 accelerated clotting time (≈ 10 min) above vancomycin and bare surfaces, demonstrating great capacity to activate the intrinsic coagulation cascade.por
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT), FEDER funds by means of Portugal 2020 Competitive Factors Operational Program (POCI) and the Portuguese Government (OE) for funding the project PEPTEX with reference POCI-01-0145-FEDER-028074. Authors also acknowledge project UID/CTM/00264/2019 of Centre for Textile Science and Technology (2C2T), funded by national funds through FCT/MCTESpor
dc.language.isoengpor
dc.publisherWileypor
dc.relationUID/CTM/00264/2019-
dc.rightsopenAccesspor
dc.subjectBiodegradable filmspor
dc.subjectPeptide LL37por
dc.subjectAntibacterial actionpor
dc.subjectAccelerated clottingpor
dc.subjectWound-healingpor
dc.subjectbiocompatibilitypor
dc.subjectbiodegradablepor
dc.subjectbioengineeringpor
dc.subjectbiomedical Applicationspor
dc.subjectfilmspor
dc.titleAntimicrobial action and clotting time of thin, hydrated poly(Vinyl Alcohol)/cellulose acetate films functionalized with LL37 for prospective wound-healing applicationspor
dc.typearticlepor
dc.peerreviewedyespor
oaire.citationIssue18por
oaire.citationVolume137por
dc.identifier.doi10.1002/app.48626por
dc.subject.fosEngenharia e Tecnologia::Engenharia dos Materiaispor
dc.subject.wosScience & Technologypor
sdum.journalJournal of Applied Polymer Sciencepor
oaire.versionAMpor
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

Files in This Item:
File Description SizeFormat 
2019.10.03_Manuscript PVA-CA black.pdf1,32 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID