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TitleFusion proteins with chromogenic and keratin binding modules
Author(s)Tinoco, Ana
Antunes, Egipto
Martins, Madalena
Gonçalves, Filipa
Gomes, Andreia C.
Silva, Carla
Cavaco-Paulo, Artur
Ribeiro, Artur
Issue dateOct-2019
PublisherNature Research
JournalScientific Reports
CitationTinoco, Ana; Antunes, Egipto; Martins, Madalena; Gonçalves, Filipa; Gomes, Andreia C.; Silva, Carla; Cavaco-Paulo, Artur; Ribeiro, Artur, Fusion proteins with chromogenic and keratin binding modules. Scientific Reports, 9(14044), 2019
Abstract(s)The present research relates to a fusion protein comprising a chromogenic blue ultramarine protein (UM) bound to a keratin-based peptide (KP). The KP-UM fusion protein explores UM chromogenic nature together with KP affinity towards hair. For the first time a fusion protein with a chromogenic nature is explored as a hair coloring agent. The KP-UM protein colored overbleached hair, being the color dependent on the formulation polarity. The protein was able to bind to the hair cuticle and even to penetrate throughout the hair fibre. Molecular dynamics studies demonstrated that the interaction between the KP-UM protein and the hair was mediated by the KP sequence. All the formulations recovered the mechanical properties of overbleached hair and KP-UM proved to be safe when tested in human keratinocytes. Although based on a chromogenic non-fluorescent protein, the KP-UM protein presented a photoswitch phenomenon, changing from chromogenic to fluorescent depending on the wavelength selected for excitation. KP-UM protein shows the potential to be incorporated in new eco-friendly cosmetic formulations for hair coloration, decreasing the use of traditional dyes and reducing its environmental impact.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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