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dc.contributor.authorMendes, Bárbara B.por
dc.contributor.authorGómez-Florit, M.por
dc.contributor.authorHamilton, Alex G.por
dc.contributor.authorDetamore, Michael S.por
dc.contributor.authorDomingues, Rui Miguel Andradepor
dc.contributor.authorReis, R. L.por
dc.contributor.authorGomes, Manuela E.por
dc.identifier.citationMendes B. B., Gómez-Florit M., Hamilton A., Detamore M., Domingues R. M. A., Reis R. L., Gomes M. E. Human platelet lysate-based nanocomposite bioink for bioprinting hierarchical fibrillar structures, Biofabrication, doi:10.1088/1758-5090/ab33e8, 2019por
dc.description.abstractThree-dimensional (3D) bioprinting holds the promise to fabricate tissue and organ substitutes for regenerative medicine. However, the lack of bioactive inks to fabricate and support functional living constructs is one of the main limitations hindering the progress of this technology. In this study, a biofunctional human-based nanocomposite bioink (HUink) composed of platelet lysate hydrogels reinforced by cellulose nanocrystals is reported. When combined with suspended bioprinting technologies, HUink allows the biofabrication of 3D freeform constructs with high resolution and integrity, mimicking the hierarchical nano-to-macro fibrillary composition of native tissues. Remarkably, HUink supports bioprinting of stem cells with high viability immediately after extrusion and over long-term cell culture without the need for additional biochemical or animal-derived media supplementation. As opposed to typical polymer-based bioinks, the pool of growth factors, cytokines and adhesion proteins in HUink boosts cell spreading and proliferation, stimulating the fast production of cell-secreted extracellular matrix. This innovative bioprinting platform with unpaired biofunctionality allows the fabrication of complex freeform cell-laden constructs that can ultimately be applied in the development of xeno-free 3D tissue models for in vitro research or to develop tissue and organ surrogates for clinical applications.por
dc.description.sponsorshipThe authors thank Hospital da Prelada (Porto, Portugal) for providing adipose tissue samples and Hospital São João (Porto, Portugal) for providing platelet concentrates. Authors acknowledge the financial support from project NORTE-010145-FEDER-000021 supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); the European Union Framework Programme for Research and Innovation HORIZON 2020, under the TEAMING Grant agreement No 739572 - The Discoveries CTR EU, Marie Skłodowska-Curie grant agreement No 706996 and European Research Council grant agreement No 726178; FCT/MCTES (Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia, e Ensino Superior) and the Fundo Social Europeu através do Programa Operacional do Capital Humano (FSE/POCH) in the framework of PhD grant PD/59/2013 - PD/BD/113807/2015 for BBM, Post-Doc grant SFRH/BPD/112459/2015 for RMD. The authors would like to thank Tram Le, Carlos Guimarães and Luca Gasperini for their support to acquire pictures and design the drawings. The authors would also like to thank Syeda Mahwish Bakht for the gelatin rhelogical data acquisiton.-
dc.publisherIOP Publishingpor
dc.rightsembargoedAccess (1 Year)por
dc.subjectPlatelet lysatepor
dc.titleHuman platelet lysate-based nanocomposite bioink for bioprinting hierarchical fibrillar structurespor
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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