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https://hdl.handle.net/1822/61269
Título: | Production of biomass-degrading enzymes by Trichoderma reesei using liquid hot water-pretreated corncob in different conditions of oxygen transfer |
Autor(es): | Michelin, Michele Mota, André Silva, Daniel P. Ruzene, Denise S. Vicente, A. A. Teixeira, J. A. |
Palavras-chave: | Cellulases Xylanases Trichoderma Autohydrolysis Bioreactor kLa k a L |
Data: | Set-2019 |
Editora: | Springer Nature |
Revista: | Bioenergy Research |
Citação: | Michelin, Michele; Mota, André; Silva, Daniel P.; Ruzene, Denise S.; Vicente, António A.; Teixeira, José A., Production of biomass-degrading enzymes by Trichoderma reesei using liquid hot water-pretreated corncob in different conditions of oxygen transfer. Bioenergy Research, 12(3), 583-592, 2019 |
Resumo(s): | Enzymatic hydrolysis accounts for 20% of the total cost in the conversion process of lignocellulosic biomass into bioethanol. Therefore, production of biomass-degrading enzymes by using lignocellulosic residue as a fermentation substrate may be an alternative to decrease the production costs. In this study, corncob (CC) has been pretreated by liquid hot water (LHW) at 200 °C for 30 min and used as inducer source for production of biomass-degrading enzymes by Trichoderma reesei MUM 97.53. The pretreatment was used to increase the cellulose content and the accessibility to lignocellulosic material. Although the filamentous fungus secreted a broad range of cellulolytic and hemicellulolytic enzymes when grown on untreated CC, higher enzyme productions were obtained when cultured on LHW-pretreated CC in a 2-L stirred tank bioreactor (STB). Besides, the effects of aeration (2 and 4 vvm) and agitation (150 and 250 rpm) rates on enzyme production were studied by submerged fermentation in a batch STB and correlated with the volumetric oxygen transfer coefficient (kLa). Maximal cellulase, xylanase, and -xylosidase productions were found at 150 rpm and 4 vvm, while the highest -glucosidase levels were obtained at 150 rpm and 2 vvm, that corresponded to kLa values of 32.50 h1 and 16.41 h1, respectively. At higher agitation, a lower enzymatic production was observed probably due to the high shear stress in the fungal hyphae. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/61269 |
DOI: | 10.1007/s12155-019-09991-8 |
ISSN: | 1939-1234 |
Versão da editora: | http://link.springer.com/journal/12155 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_51786_1.pdf | 790,5 kB | Adobe PDF | Ver/Abrir |