Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/61065

TitleDevelopment and optimization of alpha-pinene-loaded solid lipid nanoparticles (SLN) using experimental factorial design and dispersion analysis
Author(s)Zielinska, Aleksandra
Ferreira, Nuno R.
Durazzo, Alessandra
Lucarini, Massimo
Cicero, Nicola
Mamouni, Soukaina El
Silva, Amélia M.
Nowak, Izabela
Santini, Antonello
Souto, Eliana
Keywordsa-pinene
solid lipid nanoparticles
factorial design
optimization
dispersion analyzer
instability phenomena
Issue date24-Jul-2019
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
JournalMolecules
CitationZielinska, Aleksandra; Ferreira, Nuno R.; Durazzo, Alessandra; Lucarini, Massimo; Cicero, Nicola; Mamouni, Soukaina El; Silva, Amélia M.; Nowak, Izabela; Santini, Antonello; Souto, Eliana, Development and optimization of alpha-pinene-loaded solid lipid nanoparticles (SLN) using experimental factorial design and dispersion analysis. Molecules, 24(15), 2683, 2019
Abstract(s)The encapsulation of bicyclic monoterpene α-pinene into solid lipid nanoparticles (SLN) is reported using experimental factorial design, followed by high-end dispersion analyzer LUMiSizer®. This equipment allows the characterization of the α-pinene-loaded SLN instability phenomena (e.g., sedimentation, flotation or coagulation), as well as the determination of the velocity distribution in the centrifugal field and the particle size distribution. In this work, SLN were produced by hot high-pressure homogenization technique. The influence of the independent variables, surfactant and lipid ratio on the physicochemical properties of SLN, such as mean particle size (Z-Ave), polydispersity index (PDI) and zeta potential (ZP), was estimated using a 22-factorial design. The Z-Ave and PDI were analyzed by dynamic light scattering, while ZP measurements were recorded by electrophoretic light scattering. Based on the obtained results, the optimal SLN dispersion was composed of 1 wt.% of α-pinene, 4 wt.% of solid lipid (Imwitor® 900 K) and 2.5 wt.% of surfactant (Poloxamer 188), depicting 136.7 nm of Z-Ave, 0.170 of PDI and 0 mV of ZP. Furthermore, LUMISizer® has been successfully used in the stability analysis of α-pinene-loaded SLN.
TypeArticle
URIhttp://hdl.handle.net/1822/61065
DOI10.3390/molecules24152683
ISSN1420-3049
Publisher versionhttps://www.mdpi.com/journal/molecules
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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