Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/60737

TitleA textile platform using continuous aligned and textured composite microfibers to engineer tendon-to-bone interface gradient scaffolds
Author(s)Calejo, Isabel
Almeida, Raquel Carvalho Ferreira Costa
Reis, R. L.
Gomes, Manuela E.
KeywordsBiotextiles
Cell-laden microfibers
Gradient Biomaterials
Tendon-to-bone interfaces
Wet spinning
Issue date2019
PublisherWiley
JournalAdvanced Healthcare Materials
CitationCalejo I., Costa-Almeida R., Reis R. L., Gomes M. E. A Textile Platform Using Continuous Aligned and Textured Composite Microfibers to Engineer Tendon-to-Bone Interface Gradient Scaffolds, Advanced Healthcare Materials, doi:10.1002/adhm.201900200, 2019
Abstract(s)Tendon-to-bone interfaces exhibit a hierarchical multitissue transition. To replicate the progression from mineralized to nonmineralized tissue, a novel 3D fibrous scaffold is fabricated with spatial control over mineral distribution and cellular alignment. For this purpose, wet-spun continuous microfibers are produced using polycaprolactone (PCL)/ gelatin and PCL/gelatin/hydroxyapatite nano-to-microparticles (HAp). Higher extrusion rates result in aligned PCL/gelatin microfibers while, in the case of PCL/gelatin/HAp, the presence of minerals leads to a less organized structure. Biological performance using human adipose-derived stem cells (hASCs) demonstrates that topography of PCL/gelatin microfibers can induce cytoskeleton elongation, resembling native tenogenic organization. Matrix mineralization on PCL/gelatin/HAp wet-spun composite microfibers suggests the production of an osteogenic-like matrix, without external addition of osteogenic medium supplementation. As proof of concept, a 3D gradient structure is produced by assembling PCL/gelatin and PCL/gelatin/HAp microfibers, resulting in a fibrous scaffold with a continuous topographical and compositional gradient. Overall, the feasibility of wet-spinning for the generation of continuously aligned and textured microfibers is demonstrated, which can be further assembled into more complex 3D gradient structures to mimic characteristic features of tendon-to-bone interfaces.
TypeArticle
URIhttp://hdl.handle.net/1822/60737
DOI10.1002/adhm.201900200
ISSN2192-2640
Publisher versionhttps://onlinelibrary.wiley.com/doi/full/10.1002/adhm.201900200
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
19880-Calejo_et_al-2019-Advanced_Healthcare_Materials.pdf2,91 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID