Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/60578

TitleCatalytic activation of esterases by PEGylation for polyester synthesis
Author(s)Noro, Jennifer
Castro, T.
Gonçalves, Filipa
Ribeiro, Artur
Cavaco-Paulo, Artur
Silva, Carla
KeywordsPEGylation
esterases
activity
catalysis
polyester
Issue date20-May-2019
PublisherWiley-Blackwell
JournalChemcatchem
CitationJennifer Noro; Castro, T.; Gonçalves, Filipa; Ribeiro, Artur; Cavaco-Paulo, Artur; Silva, Carla, Catalytic activation of esterases by PEGylation for polyester synthesis. ChemCatChem, 11(10), 2490-2499, 2019
Abstract(s)In this work we explored PEGylation as an efficient strategy to improve esterases catalytic performance. For this, we PEGylated three esterases, namely lipase from Candida antarctica B (CALB), lipase from Thermomyces lanuginosus (TL) and cutinase from Fusarium solani pisi (CUT) and evaluated their catalytic performance by using the biosynthesis of poly(ethylene glutarate) as model reaction. After PEGylation with a 5 kDa aldehydePEG, CALB and cutinase revealed an increase of activity against pnitrophenyl butyrate hydrolysis (2fold of increase for CALB and 4fold of increase for cutinase). Unmodified and PEGylated lipase TL displayed however similar activity results. The polymerase activity of native and PEGylated esterases was also assessed. The data revealed a higher polymerase activity for the lipase TL and cutinase PEGylated forms (88 % conversion for PEGlipase TL and 34 % for PEGcutinase). Molecular dynamics were used to evaluate the effect of PEG on the geometry of the active site of enzymes with lid domain (TL and CALB). These studies corroborate the experimental data revealing a more open active site cavity for the PEGylated catalysts facilitating the catalysis.
TypeArticle
URIhttp://hdl.handle.net/1822/60578
DOI10.1002/cctc.201900451
ISSN1867-3880
e-ISSN1867-3899
Publisher versionhttp://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1867-3899
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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