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TitleApplication of fluorescence techniques to the study of protein adsorption and packing on biomaterial surfaces
Author(s)Benesch, J.
Hungerfod, G.
Suhling, K.
Tavares, C. J.
Lewitt, J. A.
Mano, J. F.
Reis, R. L.
Issue date2008
PublisherMary Ann Liebert Inc.
JournalTissue Engineering. Part A
Abstract(s)[Excerpt] The ways proteins compete for the surface of biomaterials and change conformation are believed to be important for the host response to implants. It is possible to elucidate information on packing and any induced conformational change by making use of different fluorescence techniques on fluorescently labelled proteins. Employing probe-probe resonance energy transfer (RET) allows inter and intra protein interactions to be distinguished. Homo resonance energy transfer (hRET) avoids many problems with having two different probes and means that labelling and subsequent purification can be done in one step. [...]
AccessOpen access
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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