Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/58893
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Rodrigues, Célia F. | por |
dc.contributor.author | Correia, Alexandra | por |
dc.contributor.author | Vilanova, Manuel | por |
dc.contributor.author | Henriques, Mariana | por |
dc.date.accessioned | 2019-02-04T10:55:47Z | - |
dc.date.available | 2019-02-04T10:55:47Z | - |
dc.date.issued | 2019-01-26 | - |
dc.identifier.citation | Rodrigues, Célia F.; Correia, Alexandra; Vilanova, Manuel; Henriques, Mariana, Inflammatory Cell Recruitment in Candida glabrata Biofilm Cell-Infected Mice Receiving Antifungal Chemotherapy. Journal of Clinical Medicine, 8(2), 2019 | por |
dc.identifier.issn | 2077-0383 | por |
dc.identifier.uri | https://hdl.handle.net/1822/58893 | - |
dc.description.abstract | (1) Background: Due to a high rate of antifungal resistance, Candida glabrata is one of the most prevalent Candida spp. linked to systemic candidiasis, which is particularly critical in catheterized patients. The goal of this work was to simulate a systemic infection exclusively derived from C. glabrata biofilm cells and to evaluate the effectiveness of the treatment of two echinocandinscaspofungin (Csf) and micafungin (Mcf). (2) Methods: CD1 mice were infected with 48 h-biofilm cells of C. glabrata and then treated with Csf or Mcf. After 72 h, the efficacy of each drug was evaluated to assess the organ fungal burden through colony forming units (CFU) counting. The immune cell recruitment into target organs was evaluated by flow cytometry or histopathology analysis. (3) Results: Fungal burden was found to be higher in the liver than in the kidneys. However, none of the drugs was effective in completely eradicating C. glabrata biofilm cells. At the evaluated time point, flow cytometry analysis showed a predominant mononuclear response in the spleen, which was also evident in the liver and kidneys of the infected mice, as observed by histopathology analysis. (4) Conclusions: Echinocandins do not have a significant impact on liver and kidney fungal burden, or recruited inflammatory infiltrate, when mice are intravenously (i.v.) infected with C. glabrata biofilm-grown cells. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of the Norte 2020 - Programa Operacional Regional do Norte, financially supported by project UID/EQU/00511/2019 — Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE) funded by national funds through FCT/MCTES (PIDDAC), Célia F. Rodrigues’ (SFRH/BD/93078/20130) PhD grant and M. Elisa Rodrigues (SFRH/BPD/95401/2013) post-doc grant. | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.relation | UID/EQU/00511/2019 | por |
dc.relation | UID/EQU/00511/2019 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F95401%2F2013/PT | por |
dc.rights | openAccess | por |
dc.subject | Candida glabrata | por |
dc.subject | candidemia | por |
dc.subject | echinocandins | por |
dc.subject | resistance | por |
dc.subject | biofilms | por |
dc.subject | infection | por |
dc.subject | micafungin | por |
dc.subject | caspofungin | por |
dc.subject | in vivo | por |
dc.title | Inflammatory cell recruitment in Candida glabrata biofilm cell-infected mice receiving antifungal chemotherapy | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/journal/jcm | por |
dc.comments | CEB50506 | por |
oaire.citationIssue | 2 | por |
oaire.citationConferencePlace | Switzerland | - |
oaire.citationVolume | 8 | por |
dc.date.updated | 2019-01-26T17:39:46Z | - |
dc.identifier.eissn | 2077-0383 | por |
dc.identifier.doi | 10.3390/jcm8020142 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Clinical Medicine | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_50506_1.pdf | 5,61 MB | Adobe PDF | Ver/Abrir |