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https://hdl.handle.net/1822/58816
Título: | CARB 113: Co-assembly of peptide and carbohydrate amphiphiles to generate proteoglycan mimics |
Autor(es): | Brito, A. Abul-Haija, Y. M. Soares da Costa, D. Novoa-Carballal, Ramon Reis, R. L. Ulijn, R. V. Pires, R. A. Pashkuleva, I. |
Palavras-chave: | Glycopeptides Self-assembly Supramolecular gels |
Data: | Ago-2018 |
Editora: | ACS Publications |
Citação: | Brito A., Abul-Haija Y. M., Soares da Costa D., Novoa-Carballal R., Reis R. L., Ulijn R. V., Pires R. A., Pashkuleva I. Co-assembly of peptide and carbohydrate amphiphiles to generate proteoglycan mimics, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol. 256, pp. 113-CARB, 0065-7727, 2018 |
Resumo(s): | Peptide amphiphiles (PA) have been used as building blocks that generate nanofibrous protein mimics through self-assembly under physiological conditions. These supramolecular structures are maintained by non-covalent interactions, such as, Pi-Pi stacking, hydrogen bonding and hydrophobic effects. The generated fibers can be further crosslinked via salt bridges thus forming hydrated systems that resemble the extracellular matrix (ECM) at structural and functional level. However, the proteins in the ECM are often presented as glycoconjugates such as glycoproteins and proteoglycans. Carbohydrate-modified PAs are just emerging as alternative or complementary building blocks able to generate closer supramolecular ECM mimics. Such PAs are challenging at synthetic, supramolecular and biofunctional level. Carbohydrates bear different â OH groups prompt to react and thus, different protections are needed for selective functionalization. Moreover, once conjugated to the PA, the carbohydrate moiety can alter its self-assembling capacity, as well as, the biofunctionality of the incorporated bioactive peptide. We therefore developed a simpler approach for generation of minimalistic proteoglycan mimics: co-assembly of short, aromatic PA and their carbohydrate analogues. The nanofibers generated by this approach have a PA core (e.g. fmoc-FF) and a carbohydrate shell (e.g. fmoc-glucosamine-6-phosphate or fmoc-glucosamine-6-sulfate). They present: 1) a higher mechanical performance than the PA single component systems; 2) an improved biofunctionality as demonstrated by our studies with growth factors (e.g. FGF2), lectins and cells. Peptide amphiphiles (PA) have been used as building blocks that generate nanofibrous protein mimics through self-assembly under physiological conditions. These supramolecular structures are maintained by non-covalent interactions, such as, Pi-Pi stacking, hydrogen bonding and hydrophobic effects. The generated fibers can be further crosslinked via salt bridges thus forming hydrated systems that resemble the extracellular matrix (ECM) at structural and functional level. However, the proteins in the ECM are often presented as glycoconjugates such as glycoproteins and proteoglycans. Carbohydrate-modified PAs are just emerging as alternative or complementary building blocks able to generate closer supramolecular ECM mimics. Such PAs are challenging at synthetic, supramolecular and biofunctional level. Carbohydrates bear different –OH groups prompt to react and thus, different protections are needed for selective functionalization. Moreover, once conjugated to the PA, the carbohydrate moiety can alter its self-assembling capacity, as well as, the biofunctionality of the incorporated bioactive peptide. We therefore developed a simpler approach for generation of minimalistic proteoglycan mimics: co-assembly of short, aromatic PA and their carbohydrate analogues. The nanofibers generated by this approach have a PA core (e.g. fmoc-FF) and a carbohydrate shell (e.g. fmoc-glucosamine-6-phosphate or fmoc-glucosamine-6-sulfate). They present: 1) a higher mechanical performance than the PA single component systems; 2) an improved biofunctionality as demonstrated by our studies with growth factors (e.g. FGF2), lectins and cells. Â |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/58816 |
ISSN: | 0065-7727 |
Versão da editora: | https://plan.core-apps.com/acsboston18/abstract/22b86c0c-58bc-4db9-b2a3-18abb28dd0c6 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19706-256th ACS National Meeting & Exposition.pdf | 87,57 kB | Adobe PDF | Ver/Abrir |