Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/58787

TitleInjectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration
Author(s)Silva, C. F.
Babo, Pedro Miguel Sousa
Gulino, M.
Costa, Lígia Francisca Rodrigues
Oliveira, J. M.
Silva-Correia, Joana
Domingues, Rui Miguel Andrade
Reis, R. L.
Gomes, Manuela E.
KeywordsDental pulp cells
Endodontic tissue regeneration
Growth factors-controlled release
Injectable hydrogel
Migration
Neo-angiogenesis
Issue dateSep-2018
PublisherElsevier
JournalActa Biomaterialia
CitationSilva C. F., Babo P. S., Gulino M., Costa L., Oliveira J. M., Silva-Correia J., Domingues R. M. A., Reis R. L., Gomes M. E. Injectable and tunable hyaluronic acid hydrogels releasing chemotactic and angiogenic growth factors for endodontic regeneration, Acta Biomaterialia, doi:10.1016/j.actbio.2018.07.035, 2018
Abstract(s)Bioengineered soft tissues on any meaningful scale or complexity must incorporate aspects of the functional tissue, namely a vasculature, providing cells oxygen and nutrients critical for their survival. However, the ability of tissue engineering strategies to promote a fast revascularization is critically limited. Particularly in endodontic regenerative therapies, the complicated anatomy of the root canal system, and the narrow apical access limit the supply of new blood vessels and pulp tissue ingrowth. Here we characterize the viscoelastic and microstructural properties of a class of injectable hyaluronic acid (HA) hydrogels formed in situ, reinforced with cellulose nanocrystals (CNCs) and enriched with platelet lysate (PL), and test its ability to promote cells recruitment and proangiogenic activity in vitro. The incorporation of CNCs enhanced the stability of the materials against hydrolytic and enzymatic degradation. Moreover, the release of the chemotactic and pro-angiogenic growth factors (GFs) (PDGF and VEGF) from the PL-laden hydrogels showed an improved sustained profile proportional to the amount of incorporated CNCs. The PL-laden hydrogels exhibited preferential supportive properties of encapsulated human dental pulp cells (hDPCs) in in vitro culture conditions. Finally, PL-laden hydrogels stimulated chemotactic and pro-angiogenic activity by promoting hDPCs recruitment and cell sprouting in hDPCs/human umbilical vein endothelial cell co-cultures in vitro, and in an ex vivo model. These results support the use of the combined system as a scaffold for GFs delivery and cells recruitment, thereby exhibiting great clinical potential in treating injuries in vascularized tissues.
TypeArticle
URIhttp://hdl.handle.net/1822/58787
DOI10.1016/j.actbio.2018.07.035
ISSN1742-7061
e-ISSN1878-7568
Publisher versionhttps://www.sciencedirect.com/science/article/pii/S174270611830429X
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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