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TitleEvaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications
Author(s)Carvalho, A. M.
Marques, A. P.
Silva, Tiago José Quinteiros Lopes Henriques
Reis, R. L.
KeywordsASTM Guidelines
Biologic activity
Biomedical application
Biophysical characterization
Marine biomaterials
Marine-origin collagen
Issue dateDec-2018
JournalMarine Drugs
CitationCarvalho A. M., Marques A. P., Silva T. H., Reis R. L. Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications, Marine Drugs, Vol. 16, Issue 12, pp. 495-509, doi:10.3390/md16120495, 2018
Abstract(s)Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from the skin of codfish, previously obtained as a by-product of fish industrial plants, and characterize it regarding its use as a biomaterial for biomedical application, according to American Society for Testing and Materials (ASTM) Guidelines. Collagen type I with a high degree of purity was obtained through acid-extraction, as confirmed by colorimetric assays, SDS-PAGE and amino acid composition. Thermal analysis revealed a denaturing temperature around 16 C. Moreover, collagen showed a concentration-dependent effect in metabolism and on cell adhesion of lung fibroblast MRC-5 cells. In conclusion, this study shows that collagen can be obtained from marine-origin sources, while preserving its bioactivity, supporting its use in biomedical applications.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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