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https://hdl.handle.net/1822/58769
Título: | Evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications |
Autor(es): | Carvalho, A. M. Marques, A. P. Silva, Tiago José Quinteiros Lopes Henriques Reis, R. L. |
Palavras-chave: | ASTM Guidelines Biologic activity Biomedical application Biophysical characterization Codfish Marine biomaterials Marine-origin collagen |
Data: | Dez-2018 |
Editora: | MDPI |
Revista: | Marine Drugs |
Citação: | Carvalho A. M., Marques A. P., Silva T. H., Reis R. L. Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications, Marine Drugs, Vol. 16, Issue 12, pp. 495-509, doi:10.3390/md16120495, 2018 |
Resumo(s): | Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from the skin of codfish, previously obtained as a by-product of fish industrial plants, and characterize it regarding its use as a biomaterial for biomedical application, according to American Society for Testing and Materials (ASTM) Guidelines. Collagen type I with a high degree of purity was obtained through acid-extraction, as confirmed by colorimetric assays, SDS-PAGE and amino acid composition. Thermal analysis revealed a denaturing temperature around 16 C. Moreover, collagen showed a concentration-dependent effect in metabolism and on cell adhesion of lung fibroblast MRC-5 cells. In conclusion, this study shows that collagen can be obtained from marine-origin sources, while preserving its bioactivity, supporting its use in biomedical applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/58769 |
DOI: | 10.3390/md16120495 |
ISSN: | 1660-3397 |
Versão da editora: | https://www.mdpi.com/1660-3397/16/12/495 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19686-marinedrugs-16-00495.pdf | 1,47 MB | Adobe PDF | Ver/Abrir |