Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/58729

TitleCoculture of Spheroids/2D Cell Layers Using a Miniaturized Patterned Platform as a Versatile Method to Produce Scaffold-Free Tissue Engineering Building Blocks
Author(s)Oliveira, N. M.
Martins-Cruz, C.
Oliveira, M. B.
Reis, R. L.
Mano, J. F.
Keywords3D spheroids
Coculture
Microtissues
On‐chip assessment
Stem cells
Superhydrophobic
Issue date2018
PublisherWiley
JournalAdvanced Biosystems
CitationOliveira N. M., Martins-Cruz C., Oliveira M. B., Reis R. L., Mano J. F. Coculture of Spheroids/2D Cell Layers Using a Miniaturized Patterned Platform as a Versatile Method to Produce Scaffold-Free Tissue Engineering Building Blocks, Advanced Biosystems, Vol. 2, Issue 1, pp. 1-8, doi:10.1002/adbi.201700069, 2018.
Abstract(s)A hanging drop system that allows the multiconfigurational coculture of 3D microtissues is suggested as a versatile platform to promote the formation of functional preconditioned spheroids/microtissues, namely stemâ cellâ derived tissue engineering microtissues, with potential to be applied as scaffoldâ free building blocks. Here, superhydrophobic (SH) platforms patterned with wettable regions are adapted for the production and culture of human adiposeâ derived stemâ cell spheroids under indirect coculture with 2D layers of different cell types and direct coculture setups. The versatile indirect and direct coculture setups allow the use of cell lines as soluble biomolecules â factoriesâ to continuously modulate microtissues response aspects, including their viability, cell number, and protein expression. This novel application of patterned SH platforms may find application in the massive production of modulated spheroids for the biomedical and pharmaceutical fields, with specific added value in the biofabrication of 3D constructs for tissue regeneration, as disease models, or even for organoids preparation.
TypeArticle
URIhttp://hdl.handle.net/1822/58729
DOI10.1002/adbi.201700069
ISSN2366-7478
Publisher versionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/adbi.201700069
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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