Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/56420
Título: | Yeast metabolic state identification by fiber optics spectroscopy |
Autor(es): | Castro, C. C. Silva, João S. Lopes, V. V. Martins, R. |
Palavras-chave: | Saccharomyces cerevisiae Morphology LWUV-VIS-SWNIR reflectance spectroscopy Singular value decomposition Classification |
Data: | 2009 |
Editora: | Institute for Systems and Technologies of Information, Control and Communication (INSTICC) |
Citação: | Castro, C. C.; Silva, João S.; Lopes, V. V.; Martins, R., Yeast metabolic state identification by fiber optics spectroscopy. BIOSIGNALS 2009 - Proceedings of the 2nd International Conference on Bio-Inspired Systems and Signal Processing. Porto, Portugal, Jan 14-17, 169-178, 2009. ISBN: 978-989-8111-65-4 |
Resumo(s): | In this manuscript we explore the feasibility of using LWUV-VIS-SWNIR (340 - 1100 nm) spectroscopy to classify Saccharomyces cerevisiae colony structures in YP agar and YPD agar, under different growth conditions, such as: i) no alcohol; ii) 1 % (v/v) Ethanol; iii) 1 % (v/v) 1-Propanol; iv) 1 % (v/v) 1- butanol; v) 1 % (v/v) Isopropanol; vi) 1 % (v/v) (±)-1-Phenylethanol; vii) 1 % (v/v) Isoamyl alcohol; viii) 1 % (v/v) tert-Amyl alcohol (2-Methyl-2-butanol); and ix) 1 % (v/v) Amyl alcohol. Results show that LWUV-VISSWNIR spectroscopy has the potential for yeasts metabolic state identification once the spectral signatures of colonies differs from each others, being possible to acheive 100% of classification in UV-VIS and VISSWNIR. The UV-VIS region present high discriminant information (350-450 nm), and different responses to UV excitation were obtained. Therefore, high precision is obtained because UV-VIS and VIS-NIR exhibit different kinds of information. In the future, high precisio n analytical chemistry techniques such as mass spectroscopy and molecular biology transcriptomic studies should be performed in order to understand the detailed cell metabolism and genomic phenomena that characterize the yeast colony state. |
Tipo: | Artigo em ata de conferência |
URI: | https://hdl.handle.net/1822/56420 |
ISBN: | 978-989-8111-65-4 |
DOI: | 10.5220/0001551201690178 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Artigos em Livros de Atas / Papers in Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_3019_1.pdf | 6,42 MB | Adobe PDF | Ver/Abrir |