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https://hdl.handle.net/1822/56407
Título: | Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry |
Autor(es): | Novak, J. Basarova, G. Teixeira, J. A. Vicente, A. A. |
Palavras-chave: | Cell cycle fluorescent methods proteins yeast glycogen yeast propagation |
Data: | 2007 |
Editora: | Institute of Brewing & Distilling |
Revista: | Journal of the Institute of Brewing |
Citação: | Novak, J.; Basarova, G.; Teixeira, José A.; Vicente, António A., Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry. Journal of the Institute of Brewing, 113(3), 249-255, 2007. |
Resumo(s): | The vitality and viability of industrial strains of Saccharomyces cerevisiae was monitored during pilot plant experiments simulating yeast propagation under aerobic and anaerobic conditions. Industrial wort of 12°P original gravity was used as a growth substrate for yeast propagation. The work was carried out with three widely used Czech lager yeast industrial strains: strains 2, 7 and 95. Cell cycle, cell size, granularity, glycogen content, DNA and protein content were analyzed by flow cytometry. Significantly higher specific growth rates, higher content of yeast glycogen, earlier G2/M phase cells maximum, and faster cell protein creation was observed under aerobic conditions compared to anaerobic. Strains 7 and 95 showed losses in flocculation ability after aerobic propagation compared to anaerobic propagation. Under either aerobic or strictly anaerobic conditions, only strain 2 did not show a significant loss in flocculation ability. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/56407 |
DOI: | 10.1002/j.2050-0416.2007.tb00284.x |
ISSN: | 0046-9750 |
Versão da editora: | http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2050-0416 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_48998_1.pdf | 1,27 MB | Adobe PDF | Ver/Abrir |