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|Title:||Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry|
Teixeira, J. A.
Vicente, A. A.
|Publisher:||Institute of Brewing & Distilling|
|Journal:||Journal of the Institute of Brewing|
|Citation:||Novak, J.; Basarova, G.; Teixeira, José A.; Vicente, António A., Monitoring of brewing yeast propagation under aerobic and anaerobic conditions employing flow cytometry. Journal of the Institute of Brewing, 113(3), 249-255, 2007.|
|Abstract(s):||The vitality and viability of industrial strains of Saccharomyces cerevisiae was monitored during pilot plant experiments simulating yeast propagation under aerobic and anaerobic conditions. Industrial wort of 12°P original gravity was used as a growth substrate for yeast propagation. The work was carried out with three widely used Czech lager yeast industrial strains: strains 2, 7 and 95. Cell cycle, cell size, granularity, glycogen content, DNA and protein content were analyzed by flow cytometry. Significantly higher specific growth rates, higher content of yeast glycogen, earlier G2/M phase cells maximum, and faster cell protein creation was observed under aerobic conditions compared to anaerobic. Strains 7 and 95 showed losses in flocculation ability after aerobic propagation compared to anaerobic propagation. Under either aerobic or strictly anaerobic conditions, only strain 2 did not show a significant loss in flocculation ability.|
|Appears in Collections:||CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series|