Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/56326

TitleInterleukin-6 neutralization by antibodies immobilized at the surface of polymeric nanoparticles as a therapeutic strategy for arthritic diseases
Author(s)Lima, Ana Cláudia Fernandes
Cunha, C.
Carvalho, Agostinho
Ferreira, Helena Susana Costa Machado
Neves, N. M.
KeywordsAnti-IL-6 antibody immobilization
Arthritic diseases
Biodegradable polymeric nanoparticles
IL-6 capture and neutralization
Pro-inflammatory cytokine interleukin-6
Issue dateApr-2018
PublisherACS Publications
JournalAcs Applied Materials & Interfaces
CitationLima Ana C., Cunha C., Carvalho A., Ferreira H., Neves N. M. Interleukin-6 Neutralization by Antibodies Immobilized at the Surface of Polymeric Nanoparticles as a Therapeutic Strategy for Arthritic Diseases, Acs Applied Materials & Interfaces, Vol. 10, Issue 16, pp. 13839-13850, doi:10.1021/acsami.8b01432, 2018
Abstract(s)Arthritic diseases are disabling conditions affecting millions of patients worldwide. Pro-inflammatory cytokines, particularly interleukin-6 (IL-6), plays a crucial role in inflammation and cartilage destruction. Although the beneficial effects of antibody therapy, its efficacy is limited. Therefore, this work proposes the immobilization of antibodies at the surface of biodegradable polymeric nanoparticles (NPs) to capture and neutralize IL-6. Our system is intended to protect, extend and enhance the therapeutic efficacy after delivery. Chitosanâ hyaluronic acid NPs are synthesized as a stable monodisperse population. After determining the maximum immobilization capacity (10 μg/mL), the capture ability was confirmed. Biological assays demonstrate the NPs cytocompatibility with human articular chondrocytes (hACs) and human macrophages. hACs stimulated with macrophage conditioned medium shows the beneficial role of IL-6 capture and neutralization. Biofunctionalized NPs exhibit a prolonged action and stronger efficacy than the free antibody. In conclusion, this system can be an effective and long lasting treatment for arthritic diseases.
Typearticle
URIhttp://hdl.handle.net/1822/56326
DOI10.1021/acsami.8b01432
ISSN1944-8252
Publisher versionhttps://pubs.acs.org/doi/abs/10.1021/acsami.8b01432
Peer-Reviewedyes
AccessembargoedAccess (1 Year)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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