Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/56323

TítuloBiofunctionalized lysophosphatidic acid/silk fibroin film for cornea endothelial cell regeneration
Autor(es)Choi, J. H.
Jeon, H.
Song, J. E.
Oliveira, J. M.
Reis, R. L.
Gilson, K.
Palavras-chaveCornea
Scaffolds
Silk Fibroin
cornea endothelial cells
tissue engineering
regeneration
lysophosphatidic acid
DataAbr-2018
EditoraMDPI
RevistaNanomaterials
CitaçãoChoi J. H., Jeon H., Song J. E., Oliveira J. M., Reis R. L., Gilson K. Biofunctionalized Lysophosphatidic Acid/Silk Fibroin Film for Cornea Endothelial Cell Regeneration, Nanomaterials (Basel, Switzerland), Vol. 8, Issue 5, pp. 290, doi:10.3390/nano8050290, 2018
Resumo(s)Cornea endothelial cells (CEnCs) tissue engineering is a great challenge to repair diseased or damaged CEnCs and require an appropriate biomaterial to support cell proliferation and differentiation. Biomaterials for CEnCs tissue engineering require biocompatibility, tunable biodegradability, transparency, and suitable mechanical properties. Silk fibroin-based film (SF) is known to meet these factors, but construction of functionalized graft for bioengineering of cornea is still a challenge. Herein, lysophosphatidic acid (LPA) is used to maintain and increase the specific function of CEnCs. The LPA and SF composite film (LPA/SF) was fabricated in this study. Mechanical properties and in vitro studies were performed using a rabbit model to demonstrate the characters of LPA/SF. ATR-FTIR was characterized to identify chemical composition of the films. The morphological and physical properties were performed by SEM, AFM, transparency, and contact angle. Initial cell density and MTT were performed for adhesion and cell viability in the SF and LPA/SF film. Reverse transcription polymerase chain reactions (RT-PCR) and immunofluorescence were performed to examine gene and protein expression. The results showed that films were designed appropriately for CEnCs delivery. Compared to pristine SF, LPA/SF showed higher biocompatibility, cell viability, and expression of CEnCs specific genes and proteins. These indicate that LPA/SF, a new biomaterial, offers potential benefits for CEnCs tissue engineering for regeneration.
Tipoarticle
URIhttp://hdl.handle.net/1822/56323
DOI10.3390/nano8050290
ISSN2079-4991
e-ISSN2079-4991
Versão da editorahttps://www.mdpi.com/2079-4991/8/5/290
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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