Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/56296

TitleRapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death
Author(s)Ribeiro, V. P.
Silva-Correia, J.
Gonçalves, C.
Pina, S.
Radhouani, Hajer
Montonen, Toni
Hyttinen, J.
Roy, A.
Oliveira, A. L.
Reis, R. L.
Oliveira, J. M.
Keywords3D in vitro models
Apoptosis
Enzymatically crosslinked hydrogels
Silk Fibroin
Issue dateApr-2018
PublisherPLOS
JournalPLoS ONE
CitationRibeiro V. P., Silva-Correia J., Gonçalves C., Pina S., Radhouani H., Hyttinen J., Roy A., Oliveira A. L., Reis R. L., Oliveira J. M. Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death, PLoS ONE, Vol. 13, Issue 4, pp. e0194441, doi:10.1371/journal.pone.0194441, 2018
Abstract(s)Timely and spatially-regulated injectable hydrogels, able to suppress growing tumors in response to conformational transitions of proteins, are of great interest in cancer research and treatment. Herein, we report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, and demonstrate their use as an artificial biomimetic three-dimensional (3D) matrix. The proposed SF hydrogels presented a viscoelastic nature of injectable hydrogels and spontaneous conformational changes from random coil to β-sheet conformation under physiological conditions. A human neuronal glioblastoma (U251) cell line was used for screening cell encapsulation and in vitro evaluation within the SF hydrogels. The transparent random coil SF hydrogels promoted cell viability and proliferation up to 10 days of culturing, while the crystalline SF hydrogels converted into β-sheet structure induced the formation of TUNEL-positive apoptotic cells. Therefore, this work provides a powerful tool for the investigation of the microenvironment on the programed tumor cells death, by using rapidly responsive SF hydrogels as 3D in vitro tumor models.
TypeArticle
URIhttp://hdl.handle.net/1822/56296
DOI10.1371/journal.pone.0194441
ISSN1932-6203
Publisher versionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194441
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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