Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/55390
Título: | Xylitol production from lignocellulosic whole slurry corn cob by engineered industrial Saccharomyces cerevisiae PE-2 |
Autor(es): | Sara Baptista Cunha, Joana T. Romani, Aloia Perez Domingues, Lucília |
Palavras-chave: | Xylitol production Industrial PE-2 GRE3 aldose reductase Corn cob hemicellulosic hydrolysate Presaccharification and simultaneous saccharification and fermentation (PSSF) Industrial Saccharomyces cerevisiae PE-2 Presaccharification and simultaneous saccharification and fermentation (PSSF) |
Data: | Nov-2018 |
Editora: | Elsevier 1 |
Revista: | Bioresource Technology |
Citação: | Sara Baptista; Cunha, Joana T.; Romaní, Aloia; Domingues, Lucília, Xylitol production from lignocellulosic whole slurry corn cob by engineered industrial Saccharomyces cerevisiae PE-2. Bioresource Technology, 267, 481-491, 2018 |
Resumo(s): | In this work, the industrial Saccharomyces cerevisiae PE-2 strain, presenting innate capacity for xylitol accumulation, was engineered for xylitol production by overexpression of the endogenous GRE3 gene and expression of different xylose reductases from Pichia stipitis. The best-performing GRE3-overexpressing strain was capable to produce 148.5g/L of xylitol from high xylose-containing media, with a 0.95g/g yield, and maintained close to maximum theoretical yields (0.89g/g) when tested in non-detoxified corn cob hydrolysates. Furthermore, a successful integrated strategy was developed for the production of xylitol from whole slurry corn cob in a presaccharification and simultaneous saccharification and fermentation process (15% solid loading and 36FPU) reaching xylitol yield of 0.93g/g and a productivity of 0.54g/L·h. This novel approach results in an intensified valorization of lignocellulosic biomass for xylitol production in a fully integrated process and represents an advance towards a circular economy. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/55390 |
DOI: | 10.1016/j.biortech.2018.07.068 |
ISSN: | 0960-8524 |
e-ISSN: | 1873-2976 |
Versão da editora: | http://www.journals.elsevier.com/bioresource-technology/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_47834_1.pdf | 881,31 kB | Adobe PDF | Ver/Abrir |