Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/55390

TitleXylitol production from lignocellulosic whole slurry corn cob by engineered industrial Saccharomyces cerevisiae PE-2
Author(s)Sara Baptista
Cunha, Joana T.
Romani, Aloia Perez
Domingues, Lucília
KeywordsXylitol production
Industrial PE-2
GRE3 aldose reductase
Corn cob hemicellulosic hydrolysate
Presaccharification and simultaneous saccharification and fermentation (PSSF)
Industrial Saccharomyces cerevisiae PE-2
Presaccharification and simultaneous
saccharification and fermentation (PSSF)
Issue dateNov-2018
PublisherElsevier
JournalBioresource Technology
CitationSara Baptista; Cunha, Joana T.; Romaní, Aloia; Domingues, Lucília, Xylitol production from lignocellulosic whole slurry corn cob by engineered industrial Saccharomyces cerevisiae PE-2. Bioresource Technology, 267, 481-491, 2018
Abstract(s)In this work, the industrial Saccharomyces cerevisiae PE-2 strain, presenting innate capacity for xylitol accumulation, was engineered for xylitol production by overexpression of the endogenous GRE3 gene and expression of different xylose reductases from Pichia stipitis. The best-performing GRE3-overexpressing strain was capable to produce 148.5g/L of xylitol from high xylose-containing media, with a 0.95g/g yield, and maintained close to maximum theoretical yields (0.89g/g) when tested in non-detoxified corn cob hydrolysates. Furthermore, a successful integrated strategy was developed for the production of xylitol from whole slurry corn cob in a presaccharification and simultaneous saccharification and fermentation process (15% solid loading and 36FPU) reaching xylitol yield of 0.93g/g and a productivity of 0.54g/L·h. This novel approach results in an intensified valorization of lignocellulosic biomass for xylitol production in a fully integrated process and represents an advance towards a circular economy.
TypeArticle
URIhttp://hdl.handle.net/1822/55390
DOI10.1016/j.biortech.2018.07.068
ISSN0960-8524
e-ISSN1873-2976
Publisher versionhttp://www.journals.elsevier.com/bioresource-technology/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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