Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/5498
Título: | Large-scale production of cellulose-binding domains : adsorption studies using CBD-FITC conjugates |
Autor(es): | Pinto, João Ricardo Carvalho, Joana Mota, M. Gama, F. M. |
Palavras-chave: | Adsorption Cellulose-binding domains FITC Proteolysis |
Data: | Out-2006 |
Editora: | Springer |
Revista: | Cellulose |
Citação: | "Cellulose". ISSN 0969-0239. 13:5 (Oct. 2006) 557-569. |
Resumo(s): | A method for the gram-scale production of cellulose-binding domains (CBD) through the proteolytic digestion of a commercial nzymatic preparation (Celluclast) was developed. The CBD obtained, isolated from Trichoderma reesei cellobiohydrolase I, is highly pure and heavily glycosylated. The purified peptide has a molecular weight of 8.43 kDa, comprising the binding module, a part of the linker, and about 30% glycosidic moiety. Its properties may thus be different from recombinant ones expressed in bacteria. CBDfluorescein isothiocyanate conjugates were used to study the CBD-cellulose interaction. The presence of fluorescent peptides adsorbed on crystalline and amorphous cellulose fibers suggests that amorphous regions have a higher concentration of binding sites. The adsorption is reversible, but desorption is a very slow process. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/5498 |
DOI: | 10.1007/s10570-006-9060-5 |
ISSN: | 0969-0239 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Cellulose2006-Pinto[1].pdf | 403,55 kB | Adobe PDF | Ver/Abrir |